中文摘要：本文采用液相-气相色谱串联质谱法（MS / MS）对突尼斯大麦及其制品（大麦汤和啤酒）中24种霉菌毒素进行了检测，并基于SLE 和QuEChERS萃取方法开发了一个多霉菌毒素检测程序。66%的检测样本受到霉菌毒素污染，只有一个大麦汤样本中霉菌毒素含量为6ng/g，超过了欧盟规定的最大限量（5ng/g）。其中大麦受霉菌毒素污染最严重（占62%），霉菌毒素含量范围为1.70-287.13 ng/g，霉菌毒素类型为(DON, 15AcDON, NEO, NIV, HT2, FE1 OTA, ENA, ENA1, ENB和ENB1)，其中DON毒素在啤酒、大麦汤和大麦中的发生率分别为56%、29%和23%。但是在大麦中含量最高的ENA，达到287 ng/g。啤酒和大麦汤中含量最高的分别是15AcDON (15.6 ng/g)和ENB1 (55.1 ng/g)，此外，80%的大麦汤样本显示出共生性，没有毒理学研究发现霉菌毒素接触到消费者。
外文摘要：A study on raw barley and derived products (barley soup and beers) was carried out to determine the natural presence of twenty-four mycotoxins by both liquid chromatography and gas chromatography coupled to tandem mass spectrometry (MS/MS). The developed multi-mycotoxin procedure was based on both SLE and QuEChERS extraction steps. 66% of analyzed samples presented mycotoxin contamination and only one sample, which was soup of barley (6 ng/g), exceeded the maximum level (ML) established by EU for OTA (5 ng/g). Raw barley was the most contaminated matrix (62%), which concentrations ranged from 1.70 to 287.13 ng/g) and type of detected mycotoxins (DON, 15AcDON, NEO, NIV, HT2, FE1 OTA, ENA, ENA1, ENB and ENB1). DON was the most detected mycotoxin with an incidence of 56%, 29% and 23% in beer, soup of barley and barley, respectively. However, the highest levels detected were for ENA, in raw barley with 287 ng/g. In beer and soup of barley samples, the mycotoxins with highest level were 15AcDON (15.6 ng/g) and ENB1 (55.1 ng/g), respectively. Furthermore, 80% of positive soup of barley samples showed co-occurrence. No toxicological concern was associated to mycotoxins exposure for consumers. (C) 2017 Elsevier Ltd. All rights reserved.
外文关键词：Mycotoxins, Barley, Beers, Liquid chromatography, Gas chromatography, Daily intake
作者：Juan, C.; Berrada, H.; Manes, J.; Oueslati, S.
作者单位：Univ Valencia, Lab Food Chem & Toxicol, Fac Pharm, Av Vicent Andres Estelles S-N, E-46100 Burjassot, Spain.
期刊名称：FOOD AND CHEMICAL TOXICOLOGY
期刊影响因子：3.584
出版年份：2017
出版刊次：5
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