中文摘要：本文开发了一个新型灵敏的光电化学传感器，该传感器依靠乙草胺可抑制葡萄糖氧化酶（葡萄糖氧化酶）活性的功能激活可见光活性进行乙草胺农药残留检测。因为在可见光照射下，葡萄糖氧化酶/ CS / nh2-mil-125（Ti）/二氧化钛纳米复合材料可以产生葡萄糖溶液稳定的光电流，但当遇到乙草胺，葡萄糖氧化酶活性被抑制，会导致电流下降且光强度与乙草胺浓度成反比。检测结果发现，当乙草胺浓度范围为0.02-1.0 nM时，与光电流抑制作用成正比，当乙草胺浓度范围为10-200nM时，检测限为0.003 nM (S/N = 3)。该研究是基于监测葡萄糖氧化酶活性和抑制作用的光电化学生物传感器的一次成功尝试，并显示出了其在果蔬乙草胺农药残留检测中的实际应用价值。
 
外文摘要：A new and sensitive photoelectrochemical (PEC) biosensor which is visible-light-activated was fabricated based on acetochlor's ability to inhibit glucose oxidase (GOx) activity. An NH2-MIL-125(Ti)/TiO2 nanocomposite, as a new functional material, was used for the immobilization of GOx by using chitosan (CS) as the dispersion matrix. Under visible light irradiation, the GOx/CS/NH2-MIL-125(Ti)/TiO2 nanocomposite can generate a stable photocurrent in a glucose solution. When acetochlor was added to a phosphate buffer solution containing glucose, the activity of GOx was inhibited thus causing a photocurrent drop, and the photocurrent was inversely proportional to the acetochlor concentration. A corresponding analytical method was developed. The inhibition of the photocurrent was proportional to the concentration of acetochlor in the range from 0.02 to 1.0 nM and in the range from 10 to 200 nM with a detection limit of 0.003 nM (S/N = 3). The study is a successful attempt to make a PEC biosensor based on monitoring the activity and inhibition of GOx, and shows its practical application in acetochlor detection in fruit and vegetable samples.
外文关键词：METAL-ORGANIC FRAMEWORK; INVERSE OPAL ELECTRODES; MASS-SPECTROMETRY; WATER; CDS
作者：Jin, Dangqin; Gong, Aiqin; Zhou, Hui
作者单位：Yangzhou Polytech Inst, Dept Chem Engn, Yangzhou 225127, Jiangsu, Peoples R China.
期刊名称：RSC ADVANCES
期刊影响因子：3.289
出版年份：2017
出版刊次：5
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