中文摘要：黄曲霉毒素是真菌代谢物，对食品安全造成主要威胁。虽然已经确定这些霉菌毒素能致肝癌，但它们对生殖器官的影响尚不清楚。瞬时受体电位通道(TRPs) 在人体组织，包括胎盘中广泛表达。这些通道与胎盘中的各种功能有关。胎儿及胎盘对外源性攻击特别敏感；因而怀孕期间黄曲霉毒素暴露可能导致不良后果。此前我们已证明孕晚期饲喂黄曲霉毒素B1可增加小鼠胎盘中COX-2的表达。本文中，我们通过用胎盘细胞模型JEG-3及各自的信号通路，研究黄曲霉毒素B1对COX-2的作用。结果发现暴露于这种霉菌毒素可能扰乱妊娠期由COX-2决定的生理过程
外文摘要：Aflatoxins are fungal metabolites which pose a major threat to food safety. Although these mycotoxins are established hepatocarcinogens, their effect on the reproductive organ is unknown. Transient Receptor Potential Channels (TRPs) are ubiquitously expressed in human tissues, including the placenta. These channels are associated with various functions in the placenta. The fetus and the placenta are especially sensitive to xenobiotic assault; therefore, exposure to the aflatoxins during gestation might lead to the undesirable outcome. Previously we have shown that aflatoxin B1 administered in late gestation may increase cox-2 expression in mouse placentae. In the present study, we examined the effect of aflatoxin B1 on COX-2 by using the placental cell model JEG-3 and the respective signaling pathway. In our result, COX-2 expression was induced by the mycotoxin administration. The intracellular calcium levels were also increased in cells by aflatoxin B1 treatment as little as 1 nM. Immunoblot result showed that some TRP expressions were elevated. As inflated intracellular calcium might activate MAPKs, the underlying signaling pathway was investigated. With the help of TRP-specific inhibitors, the mycotoxin appeared to increase the expression of TRPC-3 and activate PKCβ and ERK. The significance of COX-2 in pregnancy has been well established. Exposure to this mycotoxin may perturb the physiological processes dictated by COX-2 in pregnancy.
外文关键词：Aflatoxin B1；TRP；Placental cells
作者：Zhu, Y；Tan, YQ；Leung, LK
作者单位：Chinese Univ Hong Kong
期刊名称：CHEMICO-BIOLOGICAL INTERACTIONS
期刊影响因子：2.618
出版年份：2016
出版刊次：12
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