中文摘要：有人提出以干血斑（DBS）作为婴幼儿环境毒物暴露评估的潜在可行的方法。本研究中，我们验证了一种量化AFB1处理的F344小鼠及实测的人DBS样品中AFB1-赖氨酸加合物的实验方案。结果显示DBS纸片及动物和人血清样品中AFB1-赖氨酸加合物的含量具有可比性，而且DBS技术和分析方案是一个很好的评估婴幼儿群体AFB1暴露的方法。
外文摘要：Dried blood spots (DBS) were proposed as potentially viable method for exposure assessment of environmental toxicants in infant and young children. For this study, we validated an experimental protocol to quantify AFB1-lysine adduct in DBS samples of AFB1-treated F344 rats, as well as samples from human field study. Significant dose-response relationships in AFB1-lysine adduct formation were found in DBS samples of rats treated with single- and repeated-dose AFB1. AFB1-lysine levels in DBS samples were highly correlated with corresponding serum sample levels. The Person coefficients were 0.997 for the single-dose exposure, and 0.996 for the repeated-dose exposure. Levels of AFB1-lysine adduct had also good agreement between DBS and serum samples as shown by Bland-Altman plot analysis. For human field study samples (n = 36), a Pearson correlation coefficient of 0.784 was found between AFB1-lysine adduct levels of DBS and corresponding serum samples. Bland-Altman plots showed the distribution of the log differences between DBS and serum AFB1-lysine levels are within 95% confidence intervals. These results showed AFB1-lysine adduct levels in DBS cards and serum samples from animals and human samples are comparable, and the DBS technique and analytical protocol is a good means to assess AFB1 exposure in infant and children populations.
外文关键词：Aflatoxin；Dried blood spots
作者：Xue, KS；Cai, WJ；Tang, LL；Wang, JS
作者单位：Univ Georgia
期刊名称：FOOD AND CHEMICAL TOXICOLOGY
期刊影响因子：3.584
出版年份：2016
出版刊次：12
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