中文摘要：量子点DNA纳米探针是由锰掺杂硫化锌室温磷光（RTP）量子点与DNA相结合得来的。因此，本文基于荧光共振能量转移（PRET）研发了用于转基因标识序列花椰菜花叶病毒启动子35（Ca MV 35S）DNA的定量检测RTP传感器。其基本原理是通过配体交换法将单链DNA连接到量子点表面，从而构建水溶性量子点DNA纳米探针。研究发现，该探针具有良好的RTP性能，且能够很好地检测Ca MV 35 S，从而可以实现简单、快速、高效地检测转基因生物。随着目标DNA序列的增加，量子点的磷光强度会因量子点和有机淬火BHQ(2)之间的能量转移而逐渐减少。该传感器的检测限为4.03 nm，检测范围为12-300 nm，且具有高选择性。另外，该传感器与错配序列和随机序列相比，可以有效地检测目标DNA。因此，该方法是一种非常有前途的生物分析方法。
外文摘要：A QDs-DNA nano-probe was made by combining Mn-doped ZnS room-temperature phosphorescence (RTP) quantum dots (QDs) and DNA. Then an RTP sensor for quantitative detection of genetically-modified mark sequence cauliflower mosaic virus 35S promoter (Ca MV 35S) DNA was built on basis of phosphorescent resonance energy transfer (PRET). The underlying principles were that a QDs-DNA water-soluble nano-probe was built by connecting single-strand DNA to the surfaces of QDs via a ligand exchange method. This probe had good RTP performance and could well identify Ca MV 35 S. Thereby, the simple, rapid and efficient detection of genetically-modified organisms was realized. With the increase of target DNA sequence, the phosphorescent intensity of QDs was gradually reduced due to the energy transfer between QDs and the organic quencher BHQ(2). This sensor had a detection limit of 4.03 nM and a detection range of 12-300 nM. Moreover, this sensor had high selectivity. This sensor could effectively detect the target DNA compared with mismatched and random sequences. Thus, this method is very promising for biological analysis.
外文关键词：Genetically-modified organisms; DNA; Phosphorescent resonance energy transfer; Room-temperature phosphorescence quantum dots
作者：Lv, Jinzhi; Miao, Yanming; Yang, Jiajia; 等
作者单位：Shanxi Normal Univ
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：7.476
出版年份：2017
出版刊次：5
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