中文摘要：随着对转基因生物潜在健康和环境风险的关注越来越多，我们亟需研发出一个可靠的转基因大豆检测方法。本文基于CdTe量子点与多壁碳纳米管@氧化石墨烯纳米带间的均相荧光共振能量转移（FRET）构建了一个简单、敏感和有选择性的检测方法。多壁碳纳米管@氧化石墨烯纳米带可同时检测转基因大豆的双外源DNA（花椰菜花叶病毒启动子35和胭脂碱合酶终止子）。捕获DNA被相应的量子点固定，从而可获得较强的荧光信号。单链DNA探针与多壁碳纳米管@氧化石墨烯纳米带的强π-π叠加作用会因FRET过程而导致荧光强度最小。研究发现，本文所提出的方法可通过监测相关的荧光发射同时检测P35s and TNOS，且该方法具有高灵敏度。。由于该方法的可行性和可靠性，该方法可以作为日常检测转基因生物的一个潜在工具。
外文摘要：With the increasing concern of potential health and environmental risk, it is essential to develop reliable methods for transgenic soybean detection. Herein, a simple, sensitive and selective assay was constructed based on homogeneous fluorescence resonance energy transfer (FRET) between CdTe quantum dots (QDs) and multiwalled carbon nanotubes@graphene codde nanoribbons.(MWCNTs@GONRs) to form the fluorescent "on-off-on" switching for simultaneous monitoring dual target DNAs of promoter cauliflower mosaic virus 35 s (P35s) and terminator nopaline synthase (TNOS) from transgenic soybean. The capture DNAs were immobilized with corresponding QDs to obtain strong fluorescent signals (turning on). The strong pi-pi stacking interaction between single-stranded DNA (ssDNA) probes and MWCNTs@GONRs led to minimal background fluorescence due to the FRET process (turning off). The targets of P35s and TNOS were recognized by dual fluorescent probes to form double-stranded DNA (dsDNA) through the specific hybridization between target DNAs and ssDNA probes. And the dsDNA were released from the surface of MWCNTs@GONRs, which leaded the dual fluorescent probes to generate the strong fluorescent emissions (turning on). Therefore, this proposed homogeneous assay can be achieved to detect P35s and TNOS simultaneously by monitoring the relevant fluorescent emissions. Moreover, this assay can distinguish complementary and mismatched nucleic acid sequences with high sensitivity. The constructed approach has the potential to be a tool for daily detection of genetically modified organism with the merits of feasibility and reliability.
外文关键词：Fluorescence resonance energy transfer; Multiwalled carbon nanotubes@graphene oxide nanoribbons; Quantum dots; "On-Off-On" switching; Transgenic soybean
作者：Li, Yaqi; Sun, Li; Qian, Jing; 等.
作者单位：Jiangsu Univ
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：7.476
出版年份：2017
出版刊次：6
点击下载：基于CdTe量子点与多壁碳纳米管@氧化石墨烯纳米带的荧光开关传感器可同时检测转基因大豆的双外源DNA