中文摘要：为了评估转Bt基因水稻（Bt-SY63）和常规水稻对臭氧的不同敏感度，本文在臭氧环境（A-O-3）和高浓度臭氧环境（1.5 x A-O-3，E-O-3）下进行了盆栽实验研究，并对水稻的叶片气体交换、产量、稻粒要素和抗氧化酶进行了调查。在A-O-3环境下，转Bt基因水稻Bt-SY63的叶绿素含量和产量均低于常规水稻，而气孔导度、细胞内CO2浓度（CI）、Fe含量、Zn含量则高于常规水稻；尽管转Bt基因水稻Bt-SY6的抗氧化酶（包括超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT））活性要高于常规水稻，但两者的丙二醛（MDA）含量无显著差异。在E-O-3环境下，转Bt基因水稻Bt-SY63与常规水稻的光合作用、产量、稻粒要素（除了N）显著降低（PN），且转Bt基因水稻Bt-SY63的降低程度更大；转Bt基因水稻Bt-SY63与常规水稻的MDA含量分别增加30.6和23.7%。研究结果表明，在臭氧环境环境下，与常规水稻相比，外源基因的导入可能导致转Bt基因水稻Bt-SY63在生理和生长过程出现意外变化；高浓度臭氧对转Bt基因水稻Bt-SY63的损伤更严重。本研究可为转Bt基因水稻Bt-SY63的商业发行和育种策略提供有价值的基础资料。
外文摘要：To assess the different sensitivity to ozone (O-3) between transgenic Bt Shanyou63 (Bt-SY63) and its nontransgenic counterpart Shanyou63 (SY63), the leaf gas exchange, yield, grain elements, and antioxidant enzymes were investigated by performing a pot experiment under ambient O-3 concentration (A-O-3) and elevated O-3 concentration (1.5 x A-O-3, E-O-3). Under A-O-3, the chlorophyll content and yield of Bt-SY63 were significantly lower than those of SY63, whereas the stomatal conductance (Gs), cellular CO2 concentration (Ci), and Fe, Zn concentration showed the opposite trends. No significant difference was detected for malondialdehyde (MDA) content between two cultivars, although the antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities in Bt-SY63 tended to be higher than those in SY63. Exposure to E-O-3 resulted in significant reduction for photosynthesis (Pn), yield and all grain elements (except for N) concentration in both varieties, and the extents in Bt-SY63 were much greater than those in SY63. Meanwhile, significant increases for MDA content by 30.6 and 23.7% in Bt-SY63 and SY63, respectively, were detected under E-O-3. These results demonstrated that insertion of exogenous gene could induce several unintentional changes of Bt-SY63 in physiology and growth progress, compared with SY63 under ambient O-3 concentration. On the other hand, the injury of Bt-SY63 caused by elevated O-3 concentration was more severe than that of SY63. This study provided valuable baseline information for the commercial release and breeding strategies of Bt-SY63 under the projected future climate.
外文关键词：Ozone; Transgenic Bt rice; Gas exchanges; Yield center dot grain elements; Antioxidant enzymes
作者：Li, Chunhua; Zhu, Jianguo; Zeng, Qing; 等
作者单位：Chinese Acad Sci
期刊名称：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
期刊影响因子：2.76
出版年份：2017
出版刊次：5
点击下载：转Bt基因水稻和常规水稻对臭氧浓度升高的不同响应