中文摘要：转基因植物可使我们获得富集所需性状的改进基因型。然而，在育种方案中转基因植物所导入基因的稳定性是必不可少的。本文对两种抗马铃薯Y病毒（PVY）的转基因烟草杂交品种的基因遗传性进行了研究。作者在温室条件下，对连续自花授粉后代群体的转基因分离率、及对马铃薯Y病毒的抗性进行了分析。另外，本文用DAS-ELISA方法检测接种植物中是否存在PVY病毒。通过本文的研究，我们获得了转基因植物的具有强PVY抗性的F4代杂交品种。
外文摘要：Genetic transformation of plants allows us to obtain improved genotypes enriched with the desired traits. However, if transgenic lines were to be used in breeding programs the stability of inserted transgenes is essential. In the present study, we followed the inheritance of transgenes in hybrids originated from crossing two transgenic tobacco lines resistant to Potato virus Y (PVY): MN 944 LMV with the transgene containing Lettuce mosaic virus coat protein gene (LMV CP) and AC Gayed ROKY2 with PVY replicase gene (ROKY2). Progeny populations generated by successive self-pollination were analyzed with respect to the transgene segregation ratio and resistance to Potato virus Y in tests carried out under greenhouse conditions. The presence of the virus in inoculated plants was detected by DAS-ELISA method. The results demonstrated the Mendelian fashion of inheritance of transgenes which were segregated independently and stably. As a result, we obtained T-4 generation of hybrid with both transgenes stacked and which was highly resistant to PVY.
中文关键词：Resistance to Potato virus Y; Transgenic hybrids; Tobacco; Transgene inheritance
作者：Czubacka, Anna; Sacco, Ermanno; Olszak-Przybys, Hanna; 等
作者单位：Inst Soil Sci & Plant Cultivat
期刊名称：JOURNAL OF APPLIED GENETICS
期刊影响因子：1.929
出版年份：2017
出版刊次：5
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