中文摘要：转基因大豆DAS-81419-2可表达两种BT衍生蛋白Cry1Ac和Cry1F。转基因大豆DAS-81419-2是一种新型的害虫综合治理工具，且可有效控制南美洲影响大豆产量的主要鳞翅目害虫。本文于2011-2016年在巴西利用人工感染来进行转基因大豆DAS-81419-2对次要鳞翅目害虫（玉米茎蛀虫、小地虎、棉铃虫）控制性能的评价研究。九个地区的试验研究发现，与非转基因大豆相比，转基因大豆DAS-81419-2在生长和生殖阶段可显著降低由玉米茎蛀虫引起的苗期死亡率、及棉铃虫引起的食用危害；转基因大豆DAS-81419-2对小地虎有适中的活性和其他的控制策略。转基因大豆DAS-81419-2对次要鳞翅目害虫的控制性及对主要鳞翅目害虫的高效控制性表明，这种新型双蛋白技术可作为控制影响大豆产量的主要和次要鳞翅目害虫的重要工具。
外文摘要：Genetically modified soybean with transgenic event DAS-81419-2 (Conkesta (TM) technology), expresses two Bacillus thuringiensis (Bt) derived proteins, Cry1Ac and Cry1F. Event DAS-81419-2 is a proposed new Integrated Pest Management (IPM) tool with demonstrated high efficacy for controlling the primary lepidopteran pests affecting soybean production in South America. Studies were conducted in Brazil from 2011 to 2016 to assess the efficacy of DAS-81419-2 against secondary lepidopteran pests including Elasmopalpus lignosellus, Agrotis ipsilon and Helicoverpa armigera, using artificial infestations to ensure uniform pest pressure. Results from research trials across nine localities showed that compared to a non Bt isoline, DAS-81419-2 significantly reduced the seedling stage plant mortality caused by E. lignosellus and the feeding damage caused by H. armigera during both vegetative and reproductive crop growth stages. Event DAS-81419-2 showed moderate activity on A. ipsilon, and additional control tactics such as a chemical insecticide applied as a seed treatment or spray during early crop stages may be needed against this pest to provide consistently higher levels of protection in fields that are at risk of infestation. Collectively, the efficacy of soybean transgenic event DAS-81419-2 for the control of secondary lepidopteran pests, added to its high efficacy on primary lepidopteran pests suggest this new, dual protein technology will be an important tool where primary and secondary pests affect soybean production.
外文关键词：Conkesta (TM); Bacillus thuringiensis; Agrotis ipsilon; Elasmopalpus lignosellus; Helicoverpa armigera
作者：Marques, Luiz H.; Santos, Antonio C.; Castro, Boris A.; 等
作者单位：Dow AgroSci Ind Ltda
期刊名称：CROP PROTECTION
期刊影响因子：1.652
出版年份：2017
出版刊次：6
点击下载：表达Cry1F和Cry1Ac蛋白的转基因大豆DAS-81419-2控制次要鳞翅目害虫的现场评价研究