中文摘要：T-2毒素，镰刀菌产生的一种真菌代谢物，出现在一系列农产品中。鱼粉的可用性降低导致了商业水产饲料中使用谷物作为蛋白质来源的增加，这就增大了霉菌毒素污染的可能性。本研究使用血液学、生化和免疫学参数及氧化应激指标，旨在研究T-2毒素经鲤鱼摄取后的毒性。研究表明这种经饲料传播的霉菌毒素能诱导鲤鱼贫血和氧化应激并引起免疫反应的改变。
外文摘要：The T-2 toxin, a fungal metabolite produced by Fusarium molds, occurs in a range of agriculture products. Reduced availability of fish meal has led to increasing use of cereals as a source of protein in commercial aquaculture feeds, which has increased the potential for mycotoxin contamination. The purpose of this study was to investigate toxicity of T-2 toxin intake in common carp (Cyprinus carpio L.) using haematological, biochemical and immunological parameters and oxidative stress indices. In a four-week feeding trial, fish were fed a commercial diet with 5.3 mg/kg T-2 toxin added. Ingestion of contaminated diet did not lead to mortality of fish, probably due to lower feed intake. On the other hand, it significantly affected haematological variables such as haematocrit, haemoglobin, red blood cell counts leading to anemia and white blood cell counts leading to leukopenia due to lymphopenia. Plasma glucose concentration and alanine amino transferase activity showed a significant increase while triglycerides concentration decreased. Activity of ceruloplasmin was significantly decreased in plasma. Further, liver glutathione S-transferase activity was significantly increased and catalase activity decreased, in parallel with a significant increase in caudal kidney catalase activity and a decrease in glutathione peroxidase activity. Finally, lipid peroxidation (detected as malondialdehyde) was significantly increased in the liver and caudal kidney. Changes in non-specific immune response and cytokine levels in head kidney indicated immune system sensitivity to T-2 toxin. Overall, the results demonstrate that this feed-borne mycotoxin is able to induce anaemia and oxidative stress and cause changes in the immune response of common carp.
外文关键词：Mycotoxin；Trichothecenes；Fusarium；TBARS；Complement；Phagocytes
作者：Matejova, I；Faldyna, M；Modra, H；Blahova, J；Palikova, M；Markova, Z；Franc, A；Vicenova, M；Vojtek, L；Bartonkova, J；Sehonova, P；Hostovsky, M；Svobodova, Z
作者单位：Univ Vet & Pharmaceut Sci Brno
期刊名称：FISH & SHELLFISH IMMUNOLOGY
期刊影响因子：3.025
出版年份：2017
出版刊次：1
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