中文摘要：本研究旨在评估两种从大麦中分离的产霉菌毒素菌，黄曲霉和黑曲霉，在受到营养和环境因素影响时，分别产生黄曲霉毒素B1（AFB1）和赭曲霉毒素A（OTA）的能力。结果表明在全球范围内，黄曲霉和黑曲霉分别产生AFB1和OTA的最佳条件显著不同。
外文摘要：The objective of the present work was to evaluate the ability of two mycotoxigenic species Aspergillus flavus and Aspergillus niger, isolated from barley, to produce aflatoxin B1 (AFB1) and ochratoxin A (OTA), respectively, as affected by nutritional and environmental factors. Six carbon sources (D-fructose, D-glucose, D-galactose, lactose, sucrose and starch) and different water activities (0.90, 0.95 and 0.98), temperature (20 and 28 °C) and incubation time (5 and 10 days) were tested. The results showed that optimal conditions for growth on Barley Meal Extract Agar (BMEA) medium were 28 °C and 0.95 aw for A. niger strain and 28 °C and 0.98 aw for A. flavus strain. Optimal conditions for OTA and AFB1 production were largely different for the two tested strains. A. niger had an optimal OTA production at 0.98 aw and 20 °C after 10 days of incubation while A. flavus had an optimal AFB1 production at 0.95–0.98 aw and 28 °C after 5 days of incubation. These results indicates that A. flavus has a higher optimum temperature for mycotoxin synthesis than A. niger and takes greater advantage of drier conditions for maximum AFB1 production. In the current study, both OTA and AFB1 production of A. niger and A. flavus were highly influenced by carbon sources. The sugar that provided the highest toxin levels in the cultures of the two species was sucrose with the lowest levels given by starch. OTA production by A. niger was also highly induced by fructose as carbon sources, while, AFB1 production by A. flavus was favored by glucose. Globally, our results showed, significantly different optimal conditions for production of AFB1 and OTA, respectively, by A. flavus and A. niger.
外文关键词：Ochratoxin A；Aflatoxin B1；Temperature；Water activity；Carbon source
作者：Lasram, S；Hamdi, Z；Chenenaoui, S；Mliki, A；Ghorbel, A
作者单位：Borj Cedria Biotechnol Ctr
期刊名称：JOURNAL OF STORED PRODUCTS RESEARCH
期刊影响因子：1.533
出版年份：2016
出版刊次：10
点击下载：比较研究温度、水分活度和碳源对从大麦中分离的黄曲霉和黑曲霉的产毒能力的影响