中文摘要：本文介绍了一种用于同时检测果蔬中毒死蜱和丙溴磷农药残留的新型高效液相色谱-分光光度法。采用紫光分光光度法获得毒死蜱和丙溴磷的吸光度值分别为波长277 nm 和289 nm，然后采用一阶导数法进行定量。结果发现，毒死蜱和丙溴磷的校准曲线线性范围为6-16μg/mL，相关系数分别为0.9979和0.9993。采用比例为90:10、流速为1.0 mL/min的乙腈与水混合物组成流动相、219 nm洗脱液进行C-18柱高效液相分离，并且在分析前分别采用固相萃取和液液萃取法进行农药残留提取，固相萃取比液液萃取回收率效果更好。将该新型高效液相色谱-分光光度法应用于果蔬毒死蜱和丙溴磷农药残留检测，结果显示，毒死蜱残留量为0.018 mg/kg，超过了最大残留限量（0.01毫克/公斤），对身体有毒副作用，但所有果蔬样品中均为检测到丙溴磷农药残留。
外文摘要：The present work describes a novel HPLC and UV-spectrophotometric method for the simultaneous estimation of chlorpyrifos and prophenofos in vegetables and fruits. In UV method, the absorbance of chlorpyrifos and prophenofos was measured at wavelength 277 nm and 289 nm respectively and quantitation was carried out by first order derivative method. The calibration curve for chlorpyriphos and prophenofos was found to be linear in the range of 6-16 mu g/mL with correlation coefficient 0.9979 and 0.9993 respectively. The HPLC separation was achieved on Hibar C-18 column (250 x 4.6 mm, 5 mu) using mobile phase consist of acetonitrile: water (90:10, v/v) at flow rate 1.0 mL/min and the eluent was monitored at 219 nm. Pesticides were extracted from the samples by solid phase extraction and liquid-liquid extraction before the analysis. Out of these two extraction methods, solid phase extraction was found more efficient in terms of % recovery as compared to liquid-liquid extraction. The developed method was applied for estimation of chlorpyrifos and prophenofos and it was found that the chlorpyrifos was 0.018 mg/kg, which is above maximum residue limits (0.01 mg/kg), which might be toxic to body. Prophenofos was not found in any collected samples of vegetables and fruits. (C) 2017 Elsevier Ltd. All rights reserved.
 
外文关键词：Chlorpyrifos, Prophenofos, UV spectrophotometric method, HPLC, Liquid-liquid extraction, Solid phase extraction
作者：Harshit, Dave; Charmy, Kothari; Nrupesh, Patel
作者单位：Nirma Univ, Inst Pharm, Dept Pharmaceut Anal, Ahmadabad 382481, Gujarat, India.
期刊名称：Food Chemistry
期刊影响因子：4.052
出版年份：2017
出版刊次：2
点击下载：新型高效液相色谱-分光光度法测定有机磷农药残留