中文摘要：果蔬农药残留问题是影响印度农产品出口的关键问题之一，儿童农药暴露或摄入会导致关键神经系统问题，但现场量化始终没有较好地解决方案，且在ppb级别的农药残留检测一直是一个挑战，而目前除了荧光偏振免疫分析方法，没有其他方法可进行PPB级别的农药残留检测。本文采用了一个新的现场样本浓缩方法，并采用聚丙烯酸钠作为吸水材料。采用聚丙烯酸钠珠进行样本浓缩，现有的荧光偏振免疫分析系统进行PPB级别的农药残留检测，整体灵敏度可以提高10-100倍，且可在几秒内完成检测。本文分别对2,4-D、莠去津和甲基对硫磷三种杀虫剂进行了检测，检测限分别为0.1、0.5和3 ppb。本文开发了一个简单的现场荧光偏振免疫分析装置，并采用聚丙烯酸钠提高荧光偏振免疫分析灵敏度。该研究在现场定性和定量化合物分析方面具有广泛的应用潜力。
外文摘要：Pesticide residue in fruits & vegetables is one of the key issues affecting the export of rural products in India. Pesticide exposure or intake causes major nervous system problems in children. The solutions to quantitate them in field are rare and the pesticide residue detection in the parts per billion (ppb) ranges is challenging. Except ELISA, none of the existing methods can detect pesticide residues in ppb range in the field. We employed a new approach of concentrating field samples and used sodium polyacrylate (SPA) as water absorbing material. The SPA beads concentrate the field samples and obtained a sub ppb range detection using an existing FPIA system and could improve overall sensitivity by 10-100 fold. The developed assay can be done in few seconds. We have used three pesticides 2,4-D, atrazine and methyl parathion with 0.1, 0.5 and 3 ppb detection limit respectively. We developed a simple field ready FPIA device and used sodium poly acrylate (SPA) in this biochemical FPIA to enhance sensitivity. Our tests with spiked field samples offers a possibility of using SPA concentration assisted FPIA in field. This study will have far reaching applications of both qualitative & quantitative analysis chemical analytes in field samples.
 
外文关键词：FLUORESCENCE POLARIZATION IMMUNOASSAY; SODIUM POLYACRYLATE; BIOSENSOR; WATER; ORGANOPHOSPHATE; EXTRACTION; ATRAZINE
作者：Korrapati, Swathi; Pullela, Phani Kumar; Vijayalakshmi, Uthirapathy
作者单位：VIT Univ, Sch Adv Sci, Vellore 632014, Tamil Nadu, India.
期刊名称：IET NANOBIOTECHNOLOGY
期刊影响因子：1.541
出版年份：2017
出版刊次：2
点击下载：基于聚丙烯酸钠增强荧光偏振免疫分析的ppb/ppt级别的农药残留检测