中文摘要：针对日益增长的转基因生物数量的需求，高通量筛选系统是满足其检测的首选解决方案。本文成功开发了一个基于双引物寡核苷酸引物的多重转基因元素筛选系统。该系统可同时检测花椰菜花叶病毒35S（CaMV 35S）、胭脂碱合成酶基因的终止子（NOS）、玄参花叶病毒35S的启动子（FMV 35S）、新霉素磷酸转移酶基因（NPTII）、Bt cry1Ab、草丁膦乙酰转移酶基因（bar）和绿色产色链霉菌，可覆盖全球授权转基因品种的90%以上。该系统有与常规PCR等同的对退火温度的高耐受性、高特异性和检测限。本文用来自市场、国家出入境机构、参考资料和测量研究所和美国油脂化学家协会的214个样本进行了适用性研究。研究结果表明，该筛选系统适用于转基因的筛选。
外文摘要：High throughput screening systems are the preferred solution to meet the urgent requirement of increasing number of genetically modified organisms (GMOs). In this study, we have successfully developed a multiplex GMO element screening system with dual priming oligonucleotide (DPO) primers. This system can detect the cauliflower mosaic virus 35S (CaMV 35S), terminator of nopaline synthase gene (NOS), figwort mosaic virus 35S (FMV 35S) promoter, neomycin phosphotransferaseII (NPTII), Bt Cry 1Ab, phosphinothricin acetyltransferase genes (bar) and Streptomyces viridochromogenes (pat) simultaneously, which covers more than 90% of all authorized GMO species worldwide. This system exhibits a high tolerance to annealing temperatures, high specificity and a limit of detection equal to conventional PCR. A total of 214 samples from markets, national entry-exit agencies, the Institute for Reference Materials and Measurement (IRMM) and the American Oil Chemists' Society (AOCS) were also tested for applicability. This screening system is therefore suitable for GMO screening.
外文关键词：DPO primers; High-throughput; GMO screening; Wide coverage; Capillary electrophoresis
作者：Fu, Wei; Wei, Shuang; Wang, Chenguang; 等
作者单位：Chinese Acad Inspect & Quarantine
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：8
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