中文摘要：通过免疫与细胞融合获得了灵敏而特异的抗杂色曲霉素(STG) 单克隆抗体(mAb)4G10。基于该mAb开发了一种间接竞争酶联免疫试验(ic-ELISA)法和侧流免疫层析(ICA)试纸法用于检测谷物产品中的STG。研究表明，开发的这两种方法都适合大量样品的现场检测和快速筛查。
外文摘要：Sensitive and specific anti-sterigmatocystin (STG) monoclonal antibody (mAb) 4G10 was obtained by immunization and cell fusion. An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method and lateral-flow immunochromatographic assay (ICA) strip method were developed for the detection of STG in cereal products based on this mAb. The 50% inhibition concentration and limit of detection for the ic-ELISA method were 0.092 and 0.015 ng/mL, respectively. The visual limit of detection (vLOD) and cut-off value for the lateral-flow ICA strip method were 0.1 and 0.5 ng/mL, respectively. From the analysis of different cereal samples (wheat, maize and rice), the recovery rates ranged from 78.3% to 122.0% for the ic-ELISA method. For the lateral-flow ICA strip, the vLODs were 3, 1.2 and 3 ng/g, and the cut-off values were 12, 6 and 6 ng/g for wheat, maize and rice, respectively. Therefore, both of the developed methods are suitable for the on-site detection and rapid screening of numerous samples.
外文关键词：Sterigmatocystin；monoclonal antibody；ic-ELISA；lateral-flow ICA strip；cereal products
作者：Kong, DZ；Xie, ZJ；Liu, LQ；Song, SS；Kuang, H；Cui, G；Xu, CL
作者单位：Jiangnan Univ
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY
期刊影响因子：1.548
出版年份：2017
出版刊次：2
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