中文摘要：许多食品和饲料的添加剂是由转基因微生物发酵而成的。转基因枯草芽孢杆菌所生产菌株已被开发和使用于维生素B2的生产中。无论是转基因菌株还是重组DNA都不允许在欧盟市场上作为发酵产品的食品或饲料添加剂。本文对从中国进口的维生素B2产品（饲料级80%）进行了分析。作者对枯草芽孢杆菌细胞进行了鉴定，并用不同的设备（MiSeq系统, 454 or HiSeq系统）从三个全基因组测序种分离除了两种细菌DNA（LHL和LGL）。全基因组测序数据显示了氯霉素抗性基因的整合，内源性核黄素（肋骨）操纵子的缺失等。研究结果表明，食品和饲料产品中转基因菌株及其假定质粒的事件和特异实时PCR检测方法已研制成功。
外文摘要：Many food and feed additives result from fermentation of genetically modified (GM) microorganisms. For vitamin B2 (riboflavin), GM Bacillus subtilis production strains have been developed and are often used. The presence of neither the GM strain nor its recombinant DNA is allowed for fermentation products placed on the EU market as food or feed additive. A vitamin B2 product (80% feed grade) imported from China was analysed. Viable B. subtilis cells were identified and DNAs of two bacterial isolates (LHL and LGL) were subjected to three whole genome sequencing (WGS) runs with different devices (MiSeq, 454 or HiSeq system). WGS data revealed the integration of a chloramphenicol resistance gene, the deletion of the endogenous riboflavin (rib) operon and presence of four putative plasmids harbouring rib operons. Event- and construct-specific real-time PCR methods for detection of the GM strain and its putative plasmids in food and feed products have been developed.
外文关键词：Bacillus subtilis; Genetically modified organism (GMO); Genetically modified microorganisms (GMM); Next generation sequencing (NGS); Polymerase chain reaction (PCR); Detection; Vitamin B-2; Feed additives
作者：Paracchini, Valentina; Petrillo, Mauro; Reiting, Ralf; 等
作者单位：European Commiss
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：9
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