中文摘要：抗虫转基因玉米IE034已被证明是中国最具商业化的转基因玉米品种。本研究的目的是建立一个稳定可靠的定量检测方法来监测抗虫转基因玉米IE034。本文开发了一种新型实时PCR方法来对转基因玉米IE034的品系特异性进行检测。研究发现，抗虫转基因玉米IE034检测方法的检测限（LOD）约为8个起始拷贝数，定量限（LOQ）估计约40份。实时定量PCR方法的精度是从四种包含抗虫转基因玉米IE034（含量分别为5%、1%、0.5%和0.23%）的混合DNA样本筛选中证实的。量化偏差为8.7%-12.2%，相对标准偏差为2.7%-12.7%。研究结果发表，这种新型品系特异性的实时PCR方法对抗虫转基因玉米IE034及其衍生物的定量分析具有适用性和可靠性。
外文摘要：The insect-resistant transgenic maize event IE034 has been proved to be one of the most commercially developed transgenic maize events in China. This study was aimed to develop a stable and reliable quantitative detection method to monitor this new transgenic maize event. Here, we developed a novel event-specific real-time PCR method for this genetically modified maize event IE034. The resulting 134 base pair (bp) amplicon was designed according to the 5' junction of inserted sequence and flanking maize genome sequence. Standard curve of the IE034 5' event-specific sequence showed good linear regression and high PCR efficiency when using the IE034 pure line samples as calibrator. The limit of detection (LOD) for the IE034 detection method was estimated at approximately 8 initial template copies, and the limit of quantification (LOQ) was estimated at about 40 copies. The accuracy of this quantitative real-time PCR method was verified by screening four mixed DNA samples with known levels of the IE034 event (5, 1, 0.5, and 0.23 %, respectively). The quantified biases deviated from 8.7 to -12.2 %, and the relative standard deviation (RSD) ranged from 2.7 to 12.7 %. These data indicated that this new-developed IE034 event-specific real-time PCR method is suitable and reliable for the quantification of IE034 maize and its derivates.
外文关键词：Genetically modified organism; IE034 maize; Event-specific method; Real-time PCR
作者：Li, Feiwu; Long, Likun; Yan, Wei; 等
作者单位：Jilin Univ
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2017
出版刊次：6
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