中文摘要：玉米赤霉烯酮（ZEA）是一种雌激素霉菌毒素，主要是由多种镰刀菌产生的次生代谢物。先前的研究表明ZEA对家畜的繁殖不利。ZEA对哺乳动物卵巢的卵泡发育，特别是对原始卵泡形成的负面作用及其机理尚不清楚。为此，本文研究了ZEA暴露对小鼠卵巢生殖细胞囊肿破裂及原始卵泡形成的生物效应。研究表明，在体外ZEA暴露影响小鼠原始卵泡的形成。
外文摘要：Zearalenone (ZEA) is an estrogenic mycotoxin mainly produced as a secondary metabolite by numerous species of Fusarium. Previous work showed that ZEA had a negative impact on domestic animals with regard to reproduction. The adverse effects and the mechanisms of ZEA on mammalian ovarian folliculogenesis remain largely unknown, particularly its effect on primordial follicle formation. Thus, we investigated the biological effects of ZEA exposure on murine ovarian germ cell cyst breakdown and primordial follicle assembly. Our results demonstrated that newborn mouse ovaries exposed to 10 or 30μM ZEA in vitro had significantly less germ cell numbers compared to the control group. Moreover, the presence of ZEA in vitro increased the numbers of TUNEL and γH2AX positive cells within mouse ovaries and the ratio of mRNA levels of the apoptotic genes Bax/Bcl-2. Furthermore, ZEA exposure reduced the mRNA of oocyte specific genes such as LIM homeobox 8 (Lhx8), newborn ovary homeobox (Nobox), spermatogenesis and oogenesis helix-loop-helix (Sohlh2), and factor in the germline alpha (Figlα) in a dose dependent manner. Exposure to ZEA led to remarkable changes in the Lhx8 3'-UTR DNA methylation dynamics in oocytes and severely impaired folliculogenesis in ovaries after transplantation under the kidney capsules of immunodeficient mice. In conclusion, ZEA exposure impairs mouse primordial follicle formation in vitro.
外文关键词：Folliculogenesis；Primordial follicle formation；Zearalenone；Lhx8
作者：Zhang, GL；Sun, XF；Feng, YZ；Li, B；Li, YP；Yang, F；Nyachoti, CM；Shen, W；Sun, SD；Li, L
作者单位：Northwest A&F Univ
期刊名称：TOXICOLOGY AND APPLIED PHARMACOLOGY
期刊影响因子：3.847
出版年份：2017
出版刊次：2
点击下载：在体外玉米赤霉烯酮暴露通过下调Lhx8的表达损伤卵巢中原始卵泡的形成