中文摘要：黄曲霉毒素是一组毒性很高的霉菌毒素，具有高致癌力，常见于食品中。黄曲霉毒素B1（AFB1）在黄曲霉毒素家族中毒性最强。最近的研究报道AFB1能诱导自噬，但能否诱导胞外诱捕网（ETs）及先天免疫反应间的关系，活性氧(ROS)，及自噬以及ETs由AFB1诱导尚不清楚。研究数据表明AFB1诱导了MΦ ROS介导的自噬及Ets的形成以及M1表型。
外文摘要：Aflatoxins are a group of highly toxic mycotoxins with high carcinogenicity that are commonly found in foods. Aflatoxin B1 (AFB1) is the most toxic member of the aflatoxin family. A recent study reported that AFB1 can induce autophagy, but whether AFB1 can induce extracellular traps (ETs) and the relationships among innate immune responses, reactive oxygen species (ROS), and autophagy and the ETs induced by AFB1 remain unknown. Here, we demonstrated that AFB1 induced a complete autophagic process in macrophages (MΦ) (THP-1 cells and RAW264.7 cells). In addition, AFB1 induced the generation of MΦ ETs (METs) in a dose-dependent manner. In particular, the formation of METs significantly reduced the AFB1 content. Further analysis using specific inhibitors showed that the inhibition of either autophagy or ROS prevented MET formation caused by AFB1, indicating that autophagy and ROS were required for AFB1-induced MET formation. The inhibition of ROS prevented autophagy, indicating that ROS generation occurred upstream of AFB1-induced autophagy. Taken together, these data suggest that AFB1 induces ROS-mediated autophagy and ETs formation and an M1 phenotype in MΦ.
外文关键词：Aflatoxin B1；reactive oxygen species；autophagy；extracellular traps；macrophages
作者：An, YN；Shi, XC；Tang, XD；Wang, Y；Shen, FG；Zhang, QL；Wang, C；Jiang, MG；Liu, MY；Yu, L
作者单位：Jilin Univ
期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
期刊影响因子：5.218
出版年份：2017
出版刊次：2
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