中文摘要：本文描述了采用超高效液相色谱-四极杆飞行时间质谱法精确测定芒果基质中农药残留量（n=68）的方法。该方法基于乙腈-乙酸乙酯混合比为25:75（v/v）的液-液萃取法和分散固相萃取法（伯仲胺）。为了确定精确度、精密度、基质效应、检测限和定量限，对该方法进行了验证。在10、50和100 μg/kg三种浓度水平下，回收率范围为70-122%，日间和日内相对标准差低于16%。检测限和定量限范围分别为0.5-7μg/kg和2-25μg/kg。该方法线性良好、准确度和精密度较好且目标农药基质效应少。研究结果表明，该方法可用于芒果基质中农药残留分析，且成功地应用于实际样品检测。
外文摘要：This study describes an accurate mass measurement of pesticide residues (n = 68) in mango matrices by liquid chromatography coupled with quadrupole time of flight mass spectrometry (UHPLC-Q-TOF/MS). The proposed methodology is based on liquid-liquid extraction with a mixture of 25:75 (v/v) acetonitrile/ethyl acetate followed by dispersive solid phase extraction cleanup (primary secondary amine). Method validation was performed in order to establish accuracy, precision, matrix effect, limit of detection, and limit of quantification. The recoveries at three concentration levels (10, 50, and 100 mu g kg(-1)) were in the range of 70-122% with intra- and inter-day relative standard deviations which were lower than 16%. The detection and quantification limits ranged from 0.5 to 7 mu g kg(-1) and from 2 to 25 mu g kg(-1), respectively. The developed method provides good linearity, accuracy, precision, and less matrix effects for the quantitation of selected pesticides. The study confirms that this analytical method can be routinely applied for the analysis of pesticide residues in mango matrices. Finally, the validated method was successfully applied to real samples and no positive samples were found.
 
外文关键词：Pesticide residue analysis, UHPLC-Q-TOF/MS, Dual-nebulizer technique, Matrix matched calibration, Mango matrices
作者：Sivaperumal, P.; Salauddin, A.; Kumar, A. Ramesh; Santhosh, K.; Rupal, T.
作者单位：Indian Council Med Res, Natl Inst Occupat Hlth, Ahmadabad 380016, Gujarat, India.
期刊名称：Food Analytical Methods
期刊影响因子：0.0
出版年份：2017
出版刊次：7
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