中文摘要：本研究开发了一种表面等离子体共振（SPR）免疫生物传感简化耦合环境电离质谱（MS）法。研究发现，该方法对（非）预期的交叉反应分子具有快速识别的能力。
外文摘要：A simplified coupling of surface plasmon resonance (SPR) immuno-biosensing with ambient ionization mass spectrometry (MS) was developed. It combines two orthogonal analysis techniques: the biosensing capability of SPR and the chemical identification power of high resolution MS. As a proof-of-principle, deoxynivalenol (DON), an important mycotoxin, was captured using an SPR gold chip containing an antifouling layer and monoclonal antibodies against the toxin and, after washing, the chip could be taken out and analyzed by direct spray MS of the biosensor chip to confirm the identity of DON. Furthermore, cross-reacting conjugates of DON present in a naturally contaminated beer could be successfully identified, thus showing the potential of rapid identification of (un)expected cross-reacting molecules.
作者：Joshi, S；Zuilhof, H；van Beek, TA；Nielen, MWF
作者单位：Wageningen Univ & Res
期刊名称：ANALYTICAL CHEMISTRY
期刊影响因子：5.886
出版年份：2017
出版刊次：3
点击下载：生物芯片喷雾：表面等离子体共振传感与质谱简化耦合法