中文摘要：本文研究了从中国传统发酵食品中分离的植物乳杆菌减轻黄曲霉毒素B1（AFB1）的毒性的能力，及其后续解毒机制。研究发现，植物乳杆菌C88通过增加AFB1的粪便排泄，从而减轻其毒性，逆转抗氧化防御系统的缺陷，调节AFB1的代谢。
外文摘要：In this study, we investigated the potential of Lactobacillus plantarum isolated from Chinese traditional fermented foods to reduce the toxicity of aflatoxin B1 (AFB1), and its subsequent detoxification mechanism. Among all the investigated L. plantarum strains, L. plantarum C88 showed the strongest AFB1 binding capacity in vitro, and was orally administered to mice with liver oxidative damage induced by AFB1. In the therapy groups, the mice that received L. plantarum C88, especially heat-killed L. plantarum C88, after a single dose of AFB1 exposure, showed an increase in unabsorbed AFB1 in the feces. Moreover, the effects of L. plantarum C88 on the enzymes and non-enzymes antioxidant abilities in serum and liver, histological alterations of liver were assayed. The results indicated that compared to the control group, L. plantarum C88 alone administration induced significant increase of antioxidant capacity, but did not induce any significant changes in the histological picture. Compared to the mice that received AFB1 only, L. plantarum C88 treatment could weaken oxidative stress by enhancing the activity of antioxidant enzymes and elevating the expression of Glutathione S-transferase (GST) A3 through Nuclear factor erythroid (derived factor 2) related factor 2 (Nrf2) pathway. Furthermore, cytochrome P450 (CYP 450) 1A2 and CYP 3A4 expression was inhibited by L. plantarum C88, and urinary aflatoxin B1-N7-guanine (AFB-N7-guanine), a AFB1 metabolite formed by CYP 1A2 and CYP 3A4, was significantly reduced by the presence of viable L. plantarum C88. Meanwhile, the significant improvements were showed in histological pictures of the liver tissues in mice orally administered with viable L. plantarum C88. Collectively, L. plantarum C88 may alleviate AFB1 toxicity by increasing fecal AFB1 excretion, reversing deficits in antioxidant defense systems and regulating the metabolism of AFB1.
作者：Huang, L；Duan, CC；Zhao, YJ；Gao, L；Niu, CH；Xu, JB；Li, SY
作者单位：Northeast Normal Univ
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2017
出版刊次：1
点击下载：能减轻黄曲霉毒素B1毒性的植物乳杆菌C88：从中国传统发酵食品“豆腐”中分离的潜在的益生菌菌株