中文摘要：本研究提出了一种快速检测脱氧雪腐镰刀菌烯醇（DON）的方法，它基于该毒素对氧化铋修饰的丝网印刷电极(SPE)的微分脉冲伏安法（DPV）响应。研究表明该方法分析性能良好，设备为一次性用品且经济实惠，仪器便携简易，可以用于DON的原位测定。
外文摘要：In this work we propose a rapid method for deoxynivalenol (DON) detection based on the differential pulse voltammetric (DPV) response of this mycotoxin on a bismuth oxide modified screen-printed electrode (SPE). In alkaline media the reduction peak of Bi2O3 is modified and an additional peak at a more negative potential is observed due to the DON presence in solution. The experimental conditions and DPV instrumental parameters have been optimized in order to get adequate responses for DON analysis on the BiOx-SPE devices. The best results are obtained in 0.10 mol L−1 KOH using a 60 mV s−1 scan rate and 40 mV pulse amplitude. The ratio of the DON peak current to the Bi(III) reduction peak current is used for quantification purposes of this mycotoxin. Two linear response ranges (r values >0.99) with a sensitivity of 0.0019 L μg−1 (5.5×105 L mol−1) in the lower concentration range have been obtained at these measurement conditions. The reproducibility of the method is adequate, with RSD values lower than 10 % using different SPE devices for each measurement. The LOD and LOQ values (7.1 μg L−1, 2.4×10−8 mol L−1 and 24 μg L−1, 8.1×10−8 mol L−1 respectively) permit to determine DON with adequate precision and accuracy at low concentration levels. These good analytical features and the disposable and economical devices and the portable and simple instrumentation used for the measurements could make possible the implementation of this methodology for the in-situ determination of DON.
外文关键词：Bismuth oxide screen-printed electrode；Deoxynivalenol (DON)；Mycotoxin；Cyclic voltammetry；Differential pulse voltammetry
作者：Maria-Hormigos, R；Gismera, MJ；Sevilla, MT；Rumbero, A；Procopio, JR
作者单位：Univ Autonoma Madrid
期刊名称：ELECTROANALYSIS
期刊影响因子：2.471
出版年份：2017
出版刊次：1
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