中文摘要：黄曲霉毒素是一种肝脏致癌物，亟需一种检测其尿液生物标志物的快速、廉价的方法。本研究利用商业酶联免疫吸附试验（ELISA）检测了52份海地人尿液（Helica生物系统）中的AFM1。结果发现该ELISA法可以用作黄曲霉毒素暴露的流行病学的初步、定性指标。而要用它作AFM1浓度的精确、特异的指标还需要额外的精化和验证。
外文摘要：Aflatoxin is a liver carcinogen, and rapid, inexpensive methods to detect its urinary biomarkers are needed. We used a commercial enzyme-linked immuno-sorbent assay (ELISA) for aflatoxin M1 in urine (Helica Biosystems) to test 52 Haitian samples. Using this ELISA, we detected traces above the limit of detection (0.2 ng/ml urine) but below the limit of quantitation (0.4 ng/ml) in 14 samples. Liquid chromatography of all 52 Haitian urine samples revealed that only 11 had quantifiable AFM1 (mean: 29.5 pg/ml, standard error: 10.8, range: 2.94–96.5 pg/ml). The Helica ELISA may have detected forms of aflatoxin other than AFM1 in the Haitian samples, or matrix enhancement may have affected results at low AFM1 concentrations. This ELISA may serve as an initial, qualitative indicator of aflatoxin exposure for epidemiological purposes. But this method’s utility as a precise and specific indicator of AFM1 concentrations will require additional refinement and validation.
外文关键词：Aflatoxins；chemical carcinogenesis；hepatotoxicity；liver cancer；Haiti
作者：Schwartzbord, J；Severe, L；Brown, D
作者单位：Cornell Univ
期刊名称：BIOMARKERS
期刊影响因子：2.016
出版年份：2017
出版刊次：1
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