中文摘要：本文提出了一个采用高效液相色谱-荧光检测法同时检测食品中黄曲霉毒素、赭曲霉毒素A和玉米赤霉烯酮的新的预处理方法。第一步采用QuEChERS法进行提取，然后以C18固相萃取柱作为干扰去除剂进行盐析和提纯，随后将搜集的上清液进行分散液液微萃取，并采用基于中心组合设计的响应面法对微萃取过程的条件进行优化。在最优条件下获得了满意的分析性能，不同类型谷物和豆类样本的回收率范围均在63.22-107.6%，精度为0.81-8.13 %，六种真菌毒素的检测限和定量限范围分别为0.03-13μg/kg和0.22-44μg/kg。最后，通过四种标准材料(P = 0.897 > 0.05)对该方法进行了验证，该方法还成功地对当地市场的79个样本进行了检测。
外文摘要：A novel pre-treatment was proposed for the simultaneous determination of aflatoxins, ochratoxin A and zearalenone in foodstuffs using high-performance liquid chromatography with fluorescence detection. The analytical procedure was based on a first step using a quick, easy, cheap, effective, rugged, and safe based extraction procedure, followed by salting out and purification with a C18 solid-phase extraction column as interference removal clean-up. Subsequently, collected supernatantwas subjected to dispersive liquid-liquid microextraction. Response surface methodology based on central composite design was employed to optimize conditions in the microextraction procedure. Under the optimum conditions, satisfactory analytical performance with recoveries rangingfrom 63.22 to 107.6% were achieved in different types of cereals and beans, as well as desirable precisions (0.81-8.13%). Limits of detections and quantifications for these six mycotoxins ranging from0.03 to 13 mu g/ kg and 0.22 to 44 mu g/ kg, respectively, were obtained. Finally, the established method was successfully validated by four certified reference materials (P = 0.897 > 0.05) and applied to 79 samples from local markets.
 
外文关键词：central composite design, dispersive liquid-liquid microextraction, multi-mycotoxin, response surface methodology, solid-phase extraction
作者：Zhou, Jian; Xu, Jiao-Jiao; Huang, Bai-Fen; Cai, Zeng-Xuan; Ren, Yi-Ping
作者单位：Zhejiang Univ Technol, Coll Chem Engn, Hangzhou, Zhejiang, Peoples R China
期刊名称：JOURNAL OF SEPARATION SCIENCE
期刊影响因子：2.557
出版年份：2017
出版刊次：5
点击下载：采用干扰去除固相萃取结合优化分散液相微萃取法对谷物和豆类食品中多霉菌毒素进行高效液相色谱检测