中文摘要：复杂基质（比如，食物、环境和生物样本基质等）中残留物和污染物的检测通常需要多个步骤才能完成，其中至少需要集成三个独立的步骤来处理上述复杂基质：（1）采用样本制备技术从基质中分离出目标化合物。（2）采用色谱法进一步净化分离出的目标化合物。（3）采用基于光谱的色谱检测器进行定性和定量分析。上述技术在离线和在线模式下均可运行。本研究的重点是用于测定复杂基质中目标化合物的在线耦合技术。本文对这些方法的基本原理极其相关设置进行了介绍和讨论，此外还对这两种方法对生物、环境等领域的最新应用进行了回顾，重点介绍了在食品方面的分析。
 
外文摘要：The determination of residues and contaminants in complex matrices such as in the case of food, environmental, and biological samples requires a combination of several steps to succeed in the aimed goal. At least three independent steps are integrated to provide the best available situation to deal with such matrices: (1) a sample preparation technique is employed to isolate the target compounds from the rest of the matrix; (2) a chromatographic (second) step further "purifies" the isolated compounds from the co-extracted matrix interferences; (3) a spectroscopy-based device acts as chromatographic detector (ideally containing a tandem high-resolution mass analyzer) for the qualitative and quantitative analysis. These techniques can be operated in different modes including the off-line and the on-line modes. The present report focus the on-line coupling techniques aiming the determination of analytes present in complex matrices. The fundamentals of these approaches as well as the most common set ups are presented and discussed, as well as a review on the recent applications of these two approaches to the fields of bioanalytical, environmental, and food analysis are critically discussed.
 
外文关键词：In-tube solid-phase extraction, Microextraction techniques, Multidimensional separations, On-line analysis, Turbulent flow
作者：Fumes, Bruno Henrique; Andrade, Mariane Aissa; Franco, Maraissa Silva;Lancas, Fernando Mauro
作者单位：Univ Sao Paulo, Inst Chem Sao Carlos, Postal Code 780, BR-13560970 Sao Carlos, SP, Brazil.
期刊名称：JOURNAL OF SEPARATION SCIENCE
期刊影响因子：2.557
出版年份：2017
出版刊次：1
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