中文摘要：为探索小麦中霉菌毒素的早期检测，本研究联合评估了三种规范化方法（镰刀菌病的严重性、镰刀菌鉴定的PCR检测及霉菌毒素的量化）及一种新的非靶向代谢组学策略。研究的第一阶段，规范化方法能够量化霉菌毒素并鉴定镰刀菌。随后，开发了UHPLC-QTOF代谢指纹法用于研究植物致病菌的串扰。同时，证明了化学计量学分析是区分低和强感染水平的有力工具。结合这些结果，发现了与霉菌毒素的早期检测有关的串扰植物病原菌。作为对霉菌感染的快速响应发现了一种磷脂酸的过表达。相比之下，当感染增强时，显示出氧化脂类和甘油二酯的增加。
外文摘要：In order to explore the early detection of mycotoxins in wheat three standardized approaches (Fusarium disease severity, PCR assays for Fusarium spp. identification and mycotoxin quantification) and a novel untargeted metabolomics strategy were jointly assessed. In the first phase of this research, standardized approaches were able to quantify mycotoxins and identify Fusarium spp. Then, an UHPLC-QTOF metabolic fingerprinting method was developed to investigate plant-pathogen cross-talk. At the same time, chemometrics analysis demonstrated to be a powerful tool in order to distinguish low and strong infection levels. Combining these results, the cross-talk plant pathogen related to the early detection of mycotoxins was discovered. As a rapid response to fungal infection an overexpression of phosphatidic acids was discovered. By contrast, when the infection became stronger an increase of oxylipins and diacylglycerols was revealed.
外文关键词：Alkylresorcinols；Cereals；Lipidomics；Liquid chromatography-high resolution mass spectrometry；Metabolomics；Oxylipins；Plant-pathogen cross-talk；Wheat
作者：Rubert, J；Righetti, L；Stranska-Zachariasova, M；Dzuman, Z；Chrpova, J；Dall'Asta, C；Hajslova, J
作者单位：Univ Chem & Technol
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：6
点击下载：基于超高效液相色谱-质谱联用技术的非靶向代谢组学结合化学计量学：一种新的可及早发现霉菌毒素的预测工具