中文摘要：产黄曲霉毒素的霉菌可以生长在不同的食品（如谷物、熟肉）上并产生黄曲霉毒素造成污染。次生代谢产物生物合成相关基因表达的分子生物学研究需要从食品中分离出优质的霉菌RNA。本研究结合物理的细胞破碎方法及RNA纯化技术，评估了六种从不同的发霉食品中提取RNA的不同方法，获得了完整的霉菌RNA而不含这些食物的抑制剂。
外文摘要：Aflatoxin producing-moulds may grow on different foods (e.g. cereals, ripened meats) and contaminate such products with aflatoxins. Molecular studies about expression of genes involved in these secondary metabolites biosynthesis require isolation of good quality mould RNA from foods. In this work, six different RNA extraction methods, which combine physical cell disruption protocols and RNA purification techniques, were evaluated in different mouldy foods to obtain intact mould RNA and without these food inhibitors. For this, five food matrices (dry-cured ham, tea, paprika, peanut and wheat) were inoculated with Aspergillus parasiticus. Protocols that used bead beating as physical cell disruption and TRIzol yielded RNA of low quality. Differences in RNA yield were found between the two tested commercial kits. The ‘mortar-pestle RNeasy’ protocol which consists of freezing the mycelium with liquid nitrogen and grinding it with the aid of a mortar and pestle and the RNeasy kit could be utilised to obtain intact RNA from a wide range of aflatoxin-producing fungi of good quantity and quality from contaminated foods.
外文关键词：RNA isolation；Aflatoxins；Mould
作者：Bernaldez, V；Rodriguez, A；Rodriguez, M；Sanchez-Montero, L；Cordoba, JJ
作者单位：Univ Extremadura
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY
期刊影响因子：2.711
出版年份：2017
出版刊次：5
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