中文摘要：用氧化石墨烯(GO)及羧基化GO作侧流免疫分析标记，取代常规使用的胶体金和彩色乳胶标签。该方法已成功用于农产品中AFB1的现场检测，并认为它为使用GO和羧基化GO标记，通过侧流免疫分析筛查其它毒素开辟了新途径。
外文摘要：Graphene oxide (GO) and carboxylated GO were used as labels for lateral flow immunoassays, instead of the conventionally used colloidal gold and colored latex labels. A sensor is demonstrated that enables fast screening for aflatoxin B1 (AFB1) as a model analyte using the antibody-GO complex as the recognition element. The visual limit of detection and cut-off value for AFB1 are 0.3 and 1 ng/mL, respectively. It is shown that GO and carboxylated GO are viable black labels for use in lateral flow assays, one typical advantage being the saving cost (compared to the use of colloidal gold). Qualitative results are achieved within 15 min, and the analytical results were in good agreement with the reference LC MS/MS method. The method was successfully applied to the on-site determination of AFB1 in agricultural products. In our perception, it opens new possibilities for the screening of other toxins by lateral flow immunoassays using GO and carboxylated GO as labels.
外文关键词：Graphene oxide；Immunochromatographic assay；Aflatoxin B-1；Label；Screening；Food analysis
作者：Yu, L；Li, PW；Ding, XX；Zhang, Q
作者单位：Chinese Acad Agr Sci
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2017
出版刊次：4
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