中文摘要：在阿根廷，禾谷镰刀菌是小麦赤霉病的主要致病菌。这种疾病影响作物产量和粮食质量，也减少了小麦的最终用途，并造成霉菌毒素污染。本研究旨在用傅立叶变换红外（FTIR）干膜技术分析2008年生长季从阿根廷回收的34株禾谷镰刀菌与表型多样性和侵袭性有关的表型特征，并应用这种技术寻找与侵袭性相关的光谱特异性标记。总之，应用FTIR技术及简单的多因素分析，可以深刻地理解禾谷镰刀菌地方分离株的表型表征，并建立脂质含量与真菌侵袭性之间的直接关系。考虑到脂质在植物与真菌的相互作用中起主要作用，本研究结果对于镰刀菌的致病机理研究来说具有很大的吸引力。
外文摘要：

Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects crop yields and grain quality also reducing the wheat end-use, and causing mycotoxin contamination. The aim of this work was to analyze the phenotypic characteristics associated with phenotypic diversity and aggressiveness of 34 F. graminearum sensu stricto isolates recovered from Argentinean fields in the 2008 growing season using the Fourier Transform Infrared (FTIR) dried film technology. We applied this technique also to search for spectral specific markers associated with aggressiveness. The combination of FUR technology with hierarchical cluster analysis allowed us to determine that this population constitutes a highly diverse and heterogeneous group of fungi with significant phenotypic variance. Still, when the spectral features of a set of these isolates were compared against their aggressiveness, as measured by disease severity, thousand grains weight, and relative yield reduction, we found that the more aggressive'isolates were richer in lipid content. Therefore, we could define several spectroscopic markers (> C-H stretching modes in the 3000-2800 window,> C=O and -C-O vibrational modes of esters at 1765-1707 cm (-1) and 1474-900 cm (-1), respectively), mostly assigned to lipid content that could be associated with F. graminearum aggressiveness. All together, by the application of FTIR techniques and simple multivariate analyses, it was possible to gain significant insights into the phenotypic characterization of F. graminearum local isolates, and to establish the existence of a direct relationship between lipid content and fungal aggressiveness. Considering that lipids have a major role as mediators in the interaction between plants and fungi our results could represent an attractive outcome in the study of Fusarium pathogenesis.

外文关键词：Fusarium graminearum；FTIR spectroscopy；Phenotypic diversity；Aggressiveness
作者：Figoli, CB；Rojo, R；Gasoni, LA；Kikot, G；Leguizamon, M；Gamba, RR；Bosch, A；Alconada, TM
作者单位：Univ Nacl La Plata
期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
期刊影响因子：3.445
出版年份：2017
出版刊次：3
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