中文摘要：黄曲霉毒素因其产生的活性氧及继而产生的氧化损伤而具有潜在的肝毒性。蜂胶是一种蜂产品，以抗氧化能力著称。本研究旨在证明埃及蜂胶提取物(EPE)对黄曲霉毒素B1（AFB1）诱导的小鼠肝毒性的抗氧化作用。研究发现EPE能减轻黄曲霉毒素B1诱导的小鼠肝毒性。
外文摘要：Aflatoxins are potent hepatotoxic due to their role in producing reactive oxygen species and consequently peroxidative damage. Propolis is a honey bee product known for its antioxidant capacity. The aim of this study was to verify the antioxidant effect of the Egyptian propolis extract (EPE) against aflatoxin B1 (AFB1)-induced hepatotoxicity in mice. Forty eight male mice were divided: first, second and third groups were used as control receiving saline, olive oil and EPE respectively, fourth was AFB1 group, fifth and sixth received EPE post or pre AFB1 treatment, respectively. EPE was given as (0.2mg/kg) 3 times a week. AFB1 was given as a single dose (0.25μg/kg). After 2 weeks, the mice were scarified and biochemical, histopathological and immunohistochemical investigations were assessed. EPE has a high content of total phenolics and alkaloids. The inhibitory concentration 50 (IC50) value for DPPH radical scavenging was 1353.8μg/mL. Pretreatment with EPE improved AFB1-induced hepatotoxicity represented in lowering alanine transaminase, aspartate aminotransferase, alkaline phosphatase, cholesterol, triglycerides, lipid peroxidation and pro-apoptotic p53 expression to 33.48±1.98 IU/ml, 53.00±2.37 IU/ml, 123.50±2.02 IU/ml, 76.50±2.66mg/dl, 54.00±3.03mg/dl, 2.22±0.14 nmol/g and 4.31±2.1 cells/field and raising the reduced glutathione, catalase, superoxide dismutase and anti-apoptotic bcl2 expression to 3.37±1.65 nmol/g, 4.92±0.25 nmol/g, 57±0.91UI/g and 39.7±5.9 cells/field which all had non-significant differences with the control, respectively. In conclusion, EPE can attenuate aflatoxin B1-induced hepatotoxicity in
mice.
外文关键词：Liver；Toxicity；Propolis；AFB1；Antioxidant defense；p53；bcl2
作者：Alm-Eldeen, AA；Basyony, MA；Elfiky, NK；Ghalwash, MM
作者单位：Tanta Univ
期刊名称：BIOMEDICINE & PHARMACOTHERAPY
期刊影响因子：2.326
出版年份：2017
出版刊次：3
点击下载：埃及蜂胶对黄曲霉毒素B1处理的雄性小鼠肝脏抗氧化及促凋亡p53和抗凋亡bcl2表达的影响