中文摘要：赫曲霉毒素A（OTA），是害处最大、含量最多的赭曲霉毒素，化学性质稳定且普遍存在于食品中。本研究中，建立并优化了试剂消耗少而灵敏度高的基于上转换发光磁性微球（UCLMMs）的流式细胞术微球技术，用于OTA检测。研究发现，进一步改进后，不同颜色的UCLMMs有望成为多种霉菌毒素的有效检测平台。
外文摘要：Ochratoxin A (OTA), the most harmful and abundant ochratoxin, is chemically stable and commonly existed in foodstuffs. In this work, upconversion luminescent-magnetic microbeads (UCLMMs) -based cytometric bead array for OTA detection with a less reagent consumption and high sensitivity has been established and optimized. In UCLMMs, upconversion nanocrystals (UCNs) for optical code present a weak background noise and no spectral cross talk between the encoding signals and target labels under two excitation conditions to improve detection sensitivity. While the superparamagnetic Fe3O4 nanoparticles (Fe3O4 NPs) aim for rapid analysis. The results show that the developed method has a sensitivity of 9.553 ppt below HPLC with a 50-μL sample and can be completed in <2 h with good accuracy and high reproducibility. Therefore, different colors of UCLMMs will become a promising assay platform for multiple mycotoxins after further improvement.
外文关键词：Upconversion luminescent-magnetic microbeads；Facile separation；Excellent noise immunity；Cytometric bead array；Ochratoxin A；Food testing
作者：Liao, ZY；Zhang, Y；Su, L；Chang, J；Wang, HJ
作者单位：Natl Ctr Supervis & Inspect Qual Food
期刊名称：JOURNAL OF NANOPARTICLE RESEARCH
期刊影响因子：2.101
出版年份：2017
出版刊次：2
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