中文摘要：T-2毒素是谷物中广泛存在的霉菌毒素。HT-2毒素是主要的代谢产物，也是谷物中的代谢物和污染物。已经确定T-2和HT-2毒素能致癌、致畸，具有肝毒性和免疫毒性。为了降低污染风险，需要研发快速、高灵敏度且廉价的分析检测方法。本研究开发了一种T-2及HT-2毒素的高灵敏化学发光酶联免疫分析方法(CL-ELISA)，可用于实际样品中T-2及HT-2毒素的检测，而无需复杂的步骤。
外文摘要：BACKGROUND: T-2 toxin is a widely distributed mycotoxin in cereals. HT-2 toxin is the major metabolites which is metabolic and contaminant in cereals as well. T-2 toxin and HT-2 toxin has been identified as carcinogenic, hepatotoxic, teratogenic, immunotoxic toxins. To reduce the risk of contamination, a rapid, high-sensitive and inexpensive assay for the detection is required. RESULTS: In this study a high-sensitive chemiluminescent enzyme-linked immunoassay (CL-ELISA) of T-2 toxin and HT-2 toxin was developed. With the help of the chemiluminescent sbustrate, this protocol exhibited a high-sensitive character with IC50 as low as 33.28 ng mL-1 and 27.27 ng mL-1 for T-2 and HT-2, respectively. In addition, this method had no cross-reaction with other structure related mycotoxins. CONCLUSION: These results indicated that the developed CL-ELISA could be applied for the T-2 toxin and HT-2 toxin detection in actual samples without complicated steps.
外文关键词：T-2 toxin；metabolite；HT-2 toxin；high-sensitivity；CL-ELISA
作者：Li, YS；Zhang, J；Mao, X；Wu, YT；Liu, GZ；Song, LT；Li, YG；Yang, JR；You, YL；Cao, XL
作者单位：Yantai Univ
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.076
出版年份：2017
出版刊次：2
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