中文摘要：本文对两个低压和三个高压凝胶渗透色谱柱对蔬菜或动物脂肪基质中多种农药残留净化效果方面进行了比较，然后采用GC/MS和LC-MS/MS法对凝胶渗透色谱柱分馏进行分析。对五种渗透色谱柱在溶剂消耗、脂质去除、农药回收率和重复性等方面的性能进行了比较，结果发现蔬菜或动物脂肪基质主要由高分子量甘油三酸酯组成，而该方法对大多数农药都达到了很好的分馏效果。而对于含量相对高的低分子量甘油三酯（比如，牛油脂肪和棕榈仁油），五种渗透色谱柱都没有提供足够的清洗并造成了干扰和系统污染，特别是在采用GC-MS/MS法进行分析时。
外文摘要：Two low-pressure columns (Bio-Beads SX-3) and three high-pressure GPC columns were compared for clean-up of a wide range of pesticides in fatty matrices of vegetable or animal origin. The GPC fractions were analyzed by GC-MS/MS and LC-MS/MS without additional clean-up. The performance of the GPC clean-up on the five column types was compared in terms of solvent consumption, lipid removal, pesticide recovery and repeatability. It was found that for fatty matrices, mainly consisting of highmolecular weight triglycerides i.e. most vegetable oils and animal fats, good fractionation is obtained for the majority of the pesticides. On the other hand, for fats and oils containing relatively high amounts of low molecular weight triglycerides, i.e. butter fat and palm kernel oil, none of the columns provided sufficient clean-up and cause interferences and system contamination, especially in the case of GC-MS/MS analysis. For the latter case, best results in terms of lipid removal and pesticide recovery were obtained on a set (2x300 mm length) of narrow bore (7.5 mm ID) columns packed with 5 mu m PL Gel material. Column loadability is, however, much lower on that set of columns compared the other evaluated GPC columns, impairing overall method sensitivity.
外文关键词：Gel permeation chromatography, Clean-up, Fatty matrices, Pesticides, GC-MS/MS, LC-MS/MS
作者：David, Frank; Devos, Christophe; Dumont, Emmie; Yang, Zhen; Sandra, Pat;Huertas-Perez, Jose Fernando
作者单位：Res Inst Chromatog, President Kennedypk 26, B-8500 Kortrijk, Belgium.Huertas-Perez, JF (reprint author), Nestle Res Ctr, POB 44, CH-1000 Lausanne 26, Switzerland.
期刊名称：TALANTA
期刊影响因子：4.162
出版年份：2017
出版刊次：4
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