中文摘要：甜菜常受多种病毒感染而造成减产，而甜菜坏死黄脉病毒（BNYVV）是其中最重要的经济作物病毒。本文介绍了一种新的用于识别甜菜坏死黄脉病毒的逆转录环介导等温扩增法，还开发了一个简单快速（无需提取RNA）检测甜菜坏死黄脉病毒的免疫捕获（IC）RT-LAMP阵列，并将其与DAS-ELISA进行了比较。研究结果表明，免疫捕获（IC）RT-LAMP阵列是一种高度可靠的识别甜菜坏死黄脉病毒的方法。
外文摘要：Sugar beet can be infected by many different viruses that can reduce yield; beet necrotic yellow vein virus (BNYVV) is one of the most economically important viruses of this crop plant. This reportdescribes a new reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for identification of BNYVV. In addition, a novel immunocapture (IC) RT-LAMP assay for rapid and easy detection(without RNA extraction) of BNYVV was developed here and compared with DAS-ELISA and RT-LAMP assays. Our results show that the IC-RT-LAMP assay is a highly reliable alternative assay for identification of BNYVV.
外文关键词：Beet necrotic yellow vein virus (BNYVV), DAS-ELISA, IC-RT-LAMP, RT-LAMP, Sugar beet
作者：Almasi, Mohammad Amin; Almasi, Galavizh
作者单位：Islamic Azad Univ, North Tehran Branch, Young Researchers & Elites Club, Tehran, Iran.
期刊名称：ARCHIVES OF VIROLOGY
期刊影响因子：2.058
出版年份：2017
出版刊次：2
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