中文摘要：T-2毒素是一种广泛分布在谷物中的霉菌毒素，HT-2毒素是T-2毒素的主要代谢产物，同样是一种谷物污染物。T-2毒素和HT-2毒素已经被确认具有致癌、肝毒性、致畸和免疫毒性等特性，为了减少污染风险，急需一个快速高灵敏且廉价的病毒检测方法。因此，本文开发了一个用于检测T-2毒素和HT-2毒素的高灵敏化学发光免疫分析法，在化学发光底物的协助下，该方法可以分别在低至33.28 ng/mL和27.27 ng/mL浓度下检测到T-2毒素和HT-2毒素，而且，该方法与其他结构相关的真菌毒素没有交叉反应。因此，该高灵敏化学发光免疫分析法可以用于真实样本中T-2毒素和HT-2毒素检测且无复杂步骤。
外文摘要：BACKGROUND: T-2 toxin is a widely distributed mycotoxin in cereals. HT-2 toxin is the major metabolite, which is also a contaminant in cereals. T-2 toxin and HT-2 toxin have been identified as having carcinogenic, hepatotoxic, teratogenic and immunotoxic properties. To reduce the risk of contamination, a rapid, highly sensitive and inexpensive assay for the detection is required. RESULTS: In this study a high-sensitivity chemiluminescent enzyme-linked immunoassay (CL-ELISA) of T-2 toxin and HT-2 toxin was developed. With the help of the chemiluminescent substrate, this protocol showed a highly sensitive character with an IC50 as low as 33.28 ng mL(-1) and 27.27 ng mL(-1) for T-2 and HT-2, respectively. In addition, this method had no cross-reaction with other structurally related mycotoxins. CONCLUSION: These results indicated that the developed CL-ELISA could be applied for the detection of T-2 toxin and HT-2 toxin in actual samples without complicated steps. (C) 2016 Society of Chemical Industry 
外文关键词：T-2 toxin, metabolite, HT-2 toxin, high-sensitivity, CL-ELISA
作者：Li, Yanshen; Zhang, Jing; Mao, Xin; Wu, Yongtao; Liu, Gongzhen; Song, Liting; Li, Yonggang; Yang, Jianrong; You, Yanli; Cao, Xuelin
作者单位：Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China.Mao, X (reprint author), Hua Hai Property & Casualty Insurance Co Ltd, Yantai 264005, Shandong, Peoples R China.
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.463
出版年份：2017
出版刊次：2
点击下载：高灵敏化学发光免疫分析法及其在T-2毒素及其主要代谢物HT-2毒素检测方面的应用