中文摘要：埃塞俄比亚甘蓝 (埃塞俄比亚芥)是一种重要的园艺作物，在东非和南部非洲的大部分地区用作叶菜。据报道其叶片含有高浓度的促进健康的植物次生代谢物。然而，还缺乏它们对健康有益的科学知识。本研究用一种人离体肝脏模型，主要研究工厂加工对植物次生代谢产物的模式及其生物活性的影响，旨在弄清埃塞俄比亚芥预防癌症的能力。研究表明埃塞俄比亚芥预防癌症的能力正如其在体外表现出的抗黄曲霉毒素保护人体肝细胞的作用。一般来说，食用埃塞俄比亚芥能促进部分防治黄曲霉毒素病的化学预防措施。
外文摘要：Background: Ethiopian kale (Brassica carinata) is a horticulturally important crop used as leafy vegetable in large parts of East and Southern Africa. The leaves are reported to contain high concentrations of health-promoting secondary plant metabolites. However, scientific knowledge on their health benefits is scarce.
Objective: This study aimed to determine the cancer preventive potential of B. carinata using a human liver in vitro model focusing on processing effects on the pattern of secondary plant metabolites and bioactivity.
Design: B. carinata was cultivated under controlled conditions and differentially processed (raw, fermented, or cooked) after harvesting. Human liver cancer cells (HepG2) were treated with ethanolic extracts of raw or processed B. carinata leaves and analyzed for their anti-genotoxic, anti-oxidant, and cytostatic potential. Chemical analyses were carried out on glucosinolates including breakdown products, phenolic compounds, carotenoids, and chlorophyll content.
Results: Pre-treatment with B. carinata extracts concentration dependently reduced aflatoxin-induced DNA damage in the Comet assay, reduced the production of reactive oxygen species as determined by electron paramagnetic resonance spectroscopy, and induced Nrf2-mediated gene expression. Increasing extract concentrations also promoted cytostasis. Processing had a significant effect on the content of secondary plant metabolites. However, different processing methodologies did not dramatically decrease bioactivity, but enhanced the protective effect in some of the endpoints studied.
Conclusion: Our findings highlight the cancer preventive potential of B. carinata as indicated by the protection of human liver cells against aflatoxin in vitro. In general, consumption of B. carinata should be encouraged as part of chemopreventive measures to combat prevalence of aflatoxin-induced diseases.
外文关键词：Aflatoxin B1；African leafy vegetables；anti-genotoxicity；Brassicaceae；cancer chemoprevention；glucosinolates；polyphenols；secondary plant metabolites
作者：Odongo, GA；Schlotz, N；Herz, C；Hanschen, FS；Baldermann, S；Neugart, S；Trierweiler, B；Frommherz, L；Franz, CMAP；Ngwene, B；Luvonga, AW；Schreiner, M；Rohn, S；Lamy, E
作者单位：Univ Freiburg Med Ctr
期刊名称：FOOD & NUTRITION RESEARCH
期刊影响因子：3.226
出版年份：2017
出版刊次：-
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