中文摘要:黄曲霉毒素能致突变、致癌和致畸。本研究旨在弄清巴西巴拉那州马林加地区商业碾碎的干小麦受产黄曲霉毒素曲霉污染的情况。研究发现碾碎的干小麦受黄曲霉污染的情况令人担忧。
外文摘要:Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of August 2011 to January 2012, were evaluated. The enumeration analysis showed that samples had up to 273.3 CFU of molds and 133.3 CFU of aflatoxigenic Aspergillus per gram of wheat. Forty-two monosporic isolates were obtained and identified as Aspergillus flavus. The isolates were analyzed regarding their aflatoxigenic potential by culture in coconut milk agar; hydroxide vapor exposure; chromatography; and polymerase chain reaction (PCR) targeting genes that code enzymes of the aflatoxins synthesis pathway. Some of the isolates were confirmed to be aflatoxin producers and several of them presented a genetic profile of aflatoxin synthesis. The obtained results demonstrated that Bulgur wheat A. flavus contamination is concerning.
外文关键词:Aspergillus flavus;aflatoxins;Bulgur wheat;PCR.
作者:Faria, CB;dos Santos, FC;de Castro, FF;Sutil, AR;Sergio, LM;Silva, MV;Machinski, M;Barbosa-Tessmann, IP
作者单位:Univ Estadual Maringa
期刊名称:FOOD SCIENCE AND TECHNOLOGY
期刊影响因子:0.729
出版年份:2017
出版刊次:1
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