中文摘要：本研究对来自叶绿素的可调荧光量子点进行了合成和表征，金纳米粒子可以通过荧光共振能量转移有效地淬灭荧光发射，而乙酰胆碱通过丁酰胆碱酯酶水解获得的硫代胆碱，可以引起金纳米粒子聚集以及荧光共振能量转移淬灭荧光的恢复。有机磷农药会不可逆转的抑制丁酰胆碱酯酶活性，从而降低回收效果。通过评价碳量子点荧光发射强度，本文建立了有机磷农药荧光共振能量转移传感检测平台。本文以对氧磷为例进行有机磷农药残留检测，传感平台显示了对数线性关系，对氧磷残留浓度范围为0.05-50μg/l，检测限为0.05μg/l。本文还对自来水和河水等真实样本进行了检测，验证了该传感器平台的可重复性和准确性。因此，该碳量子点荧光共振能量转移传感器平台在食品和环境监测方面具有广阔的应用前景。
外文摘要：Carbon quantum dots (CQDs) obtained from natural organics attract significant attention due to the abundance of carbon sources, varieties of heteroatom doping (such as N, S, P) and good biocompatibility of precursor. In this study, tunable fluorescence emission CQDs originated from chlorophyll were synthesized and characterized. The fluorescence emission can be effectively quenched by gold nanoparticles (Au NPs) via fluorescence resonance energy transfer (FRET). Thiocholine, which was produced from acetylthiocholine (ATC) by the hydrolysis of butyrylcholinesterase (BChE), could cause the aggregation of Au NPs and the corresponding recovery of FRET-quenched fluorescence emission. The catalytic activity of BChE could be irreversibly inhibited by organophosphorus pesticides (OPs), thus, the recovery effect was reduced. By evaluating the fluorescence emission intensity of CQDs, a FRET-based sensing platform for OPs determination was established. Paraoxon was studied as an example of OPs. The sensing platform displayed a linear relationship with the logarithm of the paraoxon concentrations in the range of 0.05-50 mu g L-1 and the limit of detection (LOD) was 0.05 mu g L-1. Real sample study in tap and river water revealed that this sensing platform was repeatable and accurate. The results indicate that the OP sensor is promising for applications in food safety and environmental monitoring.
外文关键词：Gold nanoparticles, Carbon quantum dots, Fluorescence resonance energy transfer, Organophosphate pesticide, Fluorescent sensor
作者：Wu, Xiaoli; Song, Yang; Yan, Xu; Zhu, Chengzhou; Ma, Yongqiang; Du, Dan;Lin, Yuehe
作者单位：Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：7.78
出版年份：2017
出版刊次：8
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