中文摘要：本研究利用超高效液相色谱-串联质谱（UPLC-MS/MS）开发了一种可靠、快速的同时检测益智子中11种霉菌毒素的方法。并用该方法分析了44批从我国不同地区购买的益智子，在4个发霉样品中检测到了黄曲霉毒素B1、玉米赤霉烯醇、赭曲霉毒素A、伏马菌素B1和B2。
外文摘要：In this study, a reliable and fast method for the simultaneous quantitation of 11 mycotoxins in Alpinia oxyphylla was developed using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLCMS/ MS). Three different extraction procedures (solid-liquid extraction, solid-phase extraction and modified QuEChERS) were evaluated. Solid-liquid extraction was fast and easy, and also provided the best recovery rate for all mycotoxins, compared to the other extraction procedures. Some crucial factors, including extraction solvent, time and temperature, were carefully optimised. Significant matrix effects were offset using matrix-matched calibration. Under these optimised conditions, our detection approach showed a good, linear dynamic range with correlation coefficients (R-2) above 0.9958. The limit of quantification ranged from 0.1 to 20 mu g/kg. Accuracy was determined in a selected matrix using blank samples spiked with the target mycotoxins at three different concentration levels. The recoveries ranged from 60% (T-2 toxin) to 111% (HT-2 toxin), with relative standard deviation < 20%. The validated method was used to analyse 44 batches of A. oxyphylla purchased from different regions of China. Aflatoxin B1, zearalenone, ochratoxin A, fumonisin B-1 and fumonisin B-2 were detected in 4 mouldy samples.
外文关键词：Alpinia oxyphylla；UPLC-MS/MS；multiple mycotoxins；solid-liquid extraction
作者：Zhao, XS；Kong, WJ；Wang, S；Wei, JH；Yang, MH
作者单位：Chinese Acad Med Sci
期刊名称：WORLD MYCOTOXIN JOURNAL
期刊影响因子：2.091
出版年份：2017
出版刊次：1
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