中文摘要：将不产毒黄曲霉菌株用于玉米田是控制真菌产生的致癌物黄曲霉毒素污染的有效策略。本研究监测了接种96小时后的玉米粒中的18个抗产毒和不产毒黄曲霉的基因。结果表明响应不产毒菌株的玉米相关抗性基因的过表达能促成黄曲霉毒素的减少。识别显著影响黄曲霉或黄曲霉毒素积累的基因将促进抗性品种的培育。
外文摘要：The application of atoxigenic Aspergillus flavus strains in maize fields has been shown to be an effective strategy for controlling contamination of aflatoxins, potent carcinogens produced by the fungus. This study monitored the expression levels of 18 defense genes against toxigenic and atoxigenic A. flavus strains in developing maize kernels over a time course of 96 h after inoculation. A stronger upregulation of genes encoding pathogenesis-related proteins, oxidative stress-related proteins, transcriptional factors and lipoxygenases were observed in response to the atoxigenic strain. On the other side, this strain showed a significant enhanced growth in the later stages of infection, measured as copy number of the constitutive calmodulin gene. These results suggest that overexpression of maize-defense-associated genes observed in response to the atoxigenic strain could contribute to an aflatoxin reduction. The identification of genes significantly affecting the resistance to A. flavus or aflatoxin accumulation would accelerate the development of resistant cultivars.
外文关键词：Zea mays；atoxigenic strain；competition；Aspergillus flavus；defense genes；host
作者：Lanubile, A；Maschietto, V；Battilani, P；Marocco, A
作者单位：Univ Cattolica Sacro Cuore
期刊名称：JOURNAL OF PLANT INTERACTIONS
期刊影响因子：1.191
出版年份：2017
出版刊次：1
点击下载：产毒和不产毒的黄曲霉菌株感染玉米粒后诱导不同的转录特征