中文摘要：UDP-葡萄糖基转移酶(UGTs)通过糖基化由镰刀菌产生的脱氧雪腐镰刀菌烯醇（DON），有助于小麦及大麦的赤霉病(FHB)抗性。本研究采用RT-PCR克隆了大麦HvUGT14077(基因库No.GU170356.1)的七对等位基因。
外文摘要：UDP-glucosyltransferases (UGTs) contribute to Fusarium head blight (FHB) resistance of wheat and barley by glycosylating the deoxynivalenol (DON), which is produced by Fusarium fungus. In this study, seven alleles of barley HvUGT14077 (GenBank No.GU170356.1) were cloned using RT-PCR. Among them, HvUGT-10W1, which was isolated from a FHB resistant barley variety 10W1, was significantly up-regulated in young spikes after F. graminearum (F.g) inoculation. HvUGT-10W1::GFP was subcellularly located in the plasma membrane and cytoplasm of the wheat protoplasts. In vitro antifungal activity assay showed that the HvUGT-10W1 protein exerted obvious inhibition against the growth of F.g. The silencing of the HvUGT-10W1 by virus-induced gene silencing (VIGS) resulted in compromised FHB resistance of 10W1, which was shown by the increased infected colonies on the leaves. These indicated that the barley HvUGT-10W1 may also contribute to F.g resistance in barley and provided a potential candidate gene to develop transgenic barley with enhanced FHB resistance.
外文关键词：UDP-glucosyltransferase gene；Hordeum vulgare L；Fusarium head blight (FHB)；Deoxynivalenol (DON)；In vitro antifungal activity；Virus-induced gene silencing (VIGS)
作者：Xing, LP；He, LQ；Xiao, J；Chen, QG；Li, MH；Shang, Y；Zhu, YF；Chen, PD；Cao, AZ；Wang, XE
作者单位：Nanjing Agr Univ
期刊名称：PLANT MOLECULAR BIOLOGY REPORTER
期刊影响因子：2.304
出版年份：2017
出版刊次：2
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