中文摘要：脱氧雪腐镰刀菌烯醇(DON)是一种频繁出现的单端孢霉烯毒素，对人畜产生毒性效应。在猪体内，DON诱导消化及免疫系统产生变化。但对生殖系统的影响的研究很少且主要基于体外模型。本研究采用一种离休模型，旨在评估DON对猪卵泡发育各时期卵巢形态的作用。结果表明DON诱导了猪卵巢的毒性效应，影响卵泡发育并干扰生殖参数。本研究数据也表明卵巢外植体是适当的评估生殖毒性的模型。
外文摘要：Deoxynivalenol (DON) is a frequently found trichothecene mycotoxin that elicits toxic effects on humans and animals. In pigs, DON induces changes in digestive and immune systems. Effects on the reproductive system are scarce and mainly based in in vitro models. The aim of this study was to evaluate, using an ex vivo model, the effects of DON on the morphology of ovaries of pigs in all stages of follicular development. Six 5-month-old pigs were used for sampling the explants. Thirty-six explants were incubated for 48 hours in culture medium (n = 18) or medium containing 10 μM of DON (n = 18). After the incubation period, the explants were submitted to histologic and immunohistochemical (proliferating cell nuclear antigen [PCNA] expression) analysis. Histologic changes were scored, and a lesional score was established. Oocytes and follicular cells immunostained for PCNA were counted. Explants exposed to DON showed a significant increase in the lesional score (P = 0.0004) compared to control explants. The main histologic changes were degeneration of oocytes and granulosa cells, interstitial edema and pyknotic cells. DON induced a reduction in the number of normal follicles in all stages of follicular development: primordial (P = 0.005), primary (P = 0.04), and growing follicles (P = 0.04) compared to control group. Deoxynivalenol also induced a significant increase (P ≤ 0.05) in the number of pyknotic oocytes in all stages of follicular development; however, no significant change in PCNA expression in oocytes or follicular cells was observed. These results indicated that DON induces toxic effects on the ovaries, affecting follicular development and interfering with reproductive parameters on pigs. Also, the present data indicate that ovarian explants are an adequate model for assessing reproductive toxicity。
外文关键词：Follicular development；Mycotoxin；PCNA；Reproductive disorder；Toxicity
作者：Gerez, JR；Desto, SS；Bracarense, APFRL
作者单位：Univ Estadual Londrina
期刊名称：THERIOGENOLOGY
期刊影响因子：1.838
出版年份：2017
出版刊次：3
点击下载：脱氧雪腐镰刀菌烯醇诱导猪卵巢的毒性效应：一种离体方法