中文摘要：本研究用多分析物、基于微球的免疫流式细胞技术结合荧光检测法检测了转基因(GM)及近等基因玉米中镰刀菌产生的霉菌毒素的含量，平行定量测定了伏马菌素B1、脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮、T-2、赭曲霉素A及黄曲霉毒素B1。结果表明Fungi-Plex™定量免疫流式细胞技术用于目标霉菌毒素的高通量平行测定，实用性很强。
外文摘要：Levels of mycotoxins produced by Fusarium species in genetically modified  and near-isogenic maize, were determined using multi-analyte, microbead-based flow immunocytometry with fluorescence detection, for the parallel quantitative determination of fumonisin B1, deoxynivalenol, zearalenone, T-2, ochratoxin A, and aflatoxin B1. Maize varieties included the genetic events MON 810 and DAS-59122-7, and their isogenic counterparts. Cobs were artificially infested by F. verticillioides and F. proliferatum conidia, and contained F. graminearum and F. sporotrichoides natural infestation. The production of fumonisin B1 and deoxynivalenol was substantially affected in GM maize lines: F. verticillioides, with the addition of F. graminearum and F. sporotrichoides, produced significantly lower levels of fumonisin B1 (~300 mg·kg-1) in DAS-59122-7 than in its isogenic line (~580 mg·kg-1), while F. proliferatum, in addition to F. graminearum and F. sporotrichoides, produced significantly higher levels of deoxynivalenol (~18 mg·kg-1) in MON 810 than in its isogenic line (~5 mg·kg-1). Fusarium verticillioides, with F. graminearum and F. sporotrichoides, produced lower amounts of deoxynivalenol and zearalenone than F. proliferatum, with F. graminearum and F. sporotrichoides. T-2 toxin production remained unchanged when considering the maize variety. The results demonstrate the utility of the Fungi-Plex™ quantitative flow immunocytometry method, applied for the high throughput parallel determination of the target mycotoxins.
外文关键词：flow cytometry；immunoanalysis；maize；MON 810；DAS-59122-7；fumonisin B1；deoxynivalenol；zearalenone；T-2；ochratoxin A；aflatoxin B1
作者：Banati, H；Darvas, B；Feher-Toth, S；Czeh, A；Szekacs, A
作者单位：Natl Res & Innovat Ctr
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：2
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