中文摘要：镰刀菌毒素玉米赤霉烯酮（ZEN）能与植物及真菌的极性分子如糖类或硫酸盐结合。迄今为止，ZEN的这些隐蔽形式在动物体内的去向尚未阐明。为了探索ZEN复合物是否促成个体的总ZEN暴露，本研究给四头猪单次口服ZEN(10µg/kg体重)及其等摩尔量的两种植物代谢物（ZEN-14-O-β-葡萄糖苷, ZEN-16-O-β-葡萄糖苷）和一种真菌代谢物（ZEN-14-硫酸盐），采用重复测量设计。研究发现，ZEN-14-硫酸盐、ZEN-14-O-β-葡萄糖苷及ZEN-16-O-β-葡萄糖苷在猪的胃肠道中彻底水解，促成ZEN的整体毒性。
外文摘要：The Fusarium mycotoxin zearalenone (ZEN) can be conjugated with polar molecules, like sugars or sulfates, by plants and fungi. To date, the fate of these modified forms of ZEN has not yet been elucidated in animals. In order to investigate whether ZEN conjugates contribute to the total ZEN exposure of an individual, ZEN (10 µg/kg b.w.) and equimolar amounts of two of its plant metabolites (ZEN-14-O-β-glucoside, ZEN-16-O-β-glucoside) and of one fungal metabolite (ZEN-14-sulfate) were orally administered to four pigs as a single bolus using a repeated measures design. The concentrations of ZEN, its modified forms and its mammalian metabolites ZEN-14-glucuronide, α-zearalenol (α-ZEL) and α-ZEL-14-glucuronide in excreta were analyzed by high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) based methods. The biological recovery of ZEN in urine was 26% ± 10%, the total biological recovery in excreta was 40% ± 8%. Intact ZEN-14-sulfate, ZEN-14-O-β-glucoside and ZEN-16-O-β-glucoside were neither detected in urine nor in feces. After ZEN-14-sulfate application, 19% ± 5% of the administered dose was recovered in urine. In feces, no ZEN metabolites were detected. The total biological recoveries of ZEN-14-O-β-glucoside and ZEN-16-O-β-glucoside in the form of their metabolites in urine were 19% ± 11% and 13% ± 7%, respectively. The total biological recoveries in urine and feces amounted to 48% ± 7% and 34 ± 3%. An explanation for the low biological recoveries could be extensive metabolization by intestinal bacteria to yet unknown metabolites. In summary, ZEN-14-sulfate, ZEN-14-O-β-glucoside, and ZEN-16-O-β-glucoside were completely hydrolyzed in the gastrointestinal tract of swine, thus contributing to the overall toxicity of ZEN.
外文关键词：mycotoxins；masked mycotoxins；plant metabolites；bioavailability；cleavage；enzymatic hydrolysis；gastrointestinal tract；high performance liquid chromatography tandem mass spectrometry
作者：Binder, SB；Schwartz-Zimmermann, HE；Varga, E；Bichl, G；Michlmayr, H；Adam, G；Berthiller, F
作者单位：Univ Nat Resources & Life Sci
期刊影响因子：0.0
出版年份：2017
出版刊次：2
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