中文摘要：霉菌毒素是由真菌产生的有毒代谢物。对于减少食物或饲料中的霉菌毒素，生物转化是一项新兴技术，它通过微生物将毒素降解为无毒代谢物。为了监测脱氧雪腐镰刀菌烯醇（DON）的生物转化，通常使用分析工具如ELISA和液相色谱-串联质谱联用技术(LC-MS/MS)。但是，这些技术不能对可能的生物转化产物的残留毒性给出确定性的答案。为此，本研究开发了一种浮萍生物测定法，为筛选微生物培养物的脱毒能力提供了新的有价值的工具。
外文摘要：Mycotoxins are toxic metabolites produced by fungi. To mitigate mycotoxins in food or feed, biotransformation is an emerging technology in which microorganisms degrade toxins into non-toxic metabolites. To monitor deoxynivalenol (DON) biotransformation, analytical tools such as ELISA and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) are typically used. However, these techniques do not give a decisive answer about the remaining toxicity of possible biotransformation products. Hence, a bioassay using Lemna minor L. was developed. A dose-response analysis revealed significant inhibition in the growth of L. minor exposed to DON concentrations of 0.25 mg/L and higher. Concentrations above 1 mg/L were lethal for the plant. This bioassay is far more sensitive than previously described systems. The bioassay was implemented to screen microbial enrichment cultures, originating from rumen fluid, soil, digestate and activated sludge, on their biotransformation and detoxification capability of DON. The enrichment cultures originating from soil and activated sludge were capable of detoxifying and degrading 5 and 50 mg/L DON. In addition, the metabolites 3-epi-DON and the epimer of de-epoxy-DON (3-epi-DOM-1) were found as biotransformation products of both consortia. Our work provides a new valuable tool to screen microbial cultures for their detoxification capacity.
外文关键词：deoxynivalenol (DON)；Lemna minor；bioassay；biotransformation；detoxification；3-epi-DON；3-epi-de-epoxy-DON (3-epi-DOM-1)
作者：Vanhoutte, I；De Mets, L；De Boevre, M；Uka, V；Di Mavungu, JD；De Saeger, S；De Gelder, L；Audenaert, K
作者单位：Univ Ghent
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：2
点击下载：通过浮萍生物测定法评估脱氧雪腐镰刀菌烯醇（DON）的微生物解毒，经生物转化生成了3-epi-DON和3-epi-DOM-1