中文摘要：镰刀菌毒素脱氧雪腐镰刀菌烯醇（DON）及玉米赤霉烯酮（ZEN）是全球谷物中最常见的污染物，造成广泛的人畜健康的不良影响。许多环境因素能影响这些霉菌毒素的产生。本研究用响应面法(RSM)优化29种培养禾谷镰刀菌的条件，达到最大产毒量。研究表明霉菌毒素可作为常规分析的标准或化学试剂。
外文摘要：Fusarium mycotoxins deoxynivalenol (DON) and zearalenone (ZEN) are the most common contaminants in cereals worldwide, causing a wide range of adverse health effects on animals and humans. Many environmental factors can affect the production of these mycotoxins. Here, we have used response surface methodology (RSM) to optimize the Fusarium graminearum strain 29 culture conditions for maximal toxin production. Three factors, medium pH, incubation temperature and time, were optimized using a Box-Behnken design (BBD). The optimized conditions for DON production were pH 4.91 and an incubation temperature of 23.75 °C for 28 days, while maximal ZEN production required pH 9.00 and an incubation temperature of 15.05 °C for 28 days. The maximum levels of DON and ZEN production were 2811.17 ng/mL and 23789.70 ng/mL, respectively. Considering the total level of DON and ZEN, desirable yields of the mycotoxins were still obtained with medium pH of 6.86, an incubation temperature of 17.76 °C and a time of 28 days. The corresponding experimental values, from the validation experiments, fitted well with these predictions. This suggests that RSM could be used to optimize Fusarium mycotoxin levels, which are further purified for use as potential mycotoxin standards. Furthermore, it shows that acidic pH is a determinant for DON production, while an alkaline environment and lower temperature (approximately 15 °C) are favorable for ZEN accumulation. After extraction, separation and purification processes, the isolated mycotoxins were obtained through a simple purification process, with desirable yields, and acceptable purity. The mycotoxins could be used as potential analytical standards or chemical reagents for routine analysis.
外文关键词：Fusarium graminearum；deoxynivalenol；zearalenone；response surface methodology；optimization；purification
作者：Wu, L；Qiu, LJ；Zhang, HJ；Sun, J；Hu, XX；Wang, BJ
作者单位：Chinese Acad Agr Sci
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：2
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