中文摘要：镰刀菌能产生大量的次生代谢物，包括非核糖体毒素白僵菌素（BEA）和恩镰孢菌素（ENNs）。两者都是由多功能酶恩镰孢菌素合成酶(ESYN1)合成，该酶兼具肽合成酶及S-腺苷L-甲硫氨酸依赖的N-甲基转移酶的活性。许多镰刀菌能产生ENNs、EBA或两者兼有，但导致这些代谢差异的机制尚不清楚。本研究通过对不同镰刀菌的esyn1进行转录测序，分析了ESYN1的一级结构，并通过超高效液相色谱-光电二极管阵列和ACQUITY QDA质量检测器（UPLC-PDA-QDa）测试了ENNs 和BEA的产生。研究发现氨基酸腺苷酸域β链的天冬酰胺789-丙氨酸793和组氨酸797-天冬酰胺802 部分能将产BEA/ENN 的镰刀菌株从仅产生ENN的菌株中区分开来。
外文摘要：Members of the fungal genus Fusarium can produce numerous secondary metabolites, including the nonribosomal mycotoxins beauvericin (BEA) and enniatins (ENNs). Both mycotoxins are synthesized by the multifunctional enzyme enniatin synthetase (ESYN1) that contains both peptide synthetase and S-adenosyl-l-methionine-dependent N-methyltransferase activities. Several Fusarium species can produce ENNs, BEA or both, but the mechanism(s) enabling these differential metabolic profiles is unknown. In this study, we analyzed the primary structure of ESYN1 by sequencing esyn1 transcripts from different Fusarium species. We measured ENNs and BEA production by ultra-performance liquid chromatography coupled with photodiode array and Acquity QDa mass detector (UPLC-PDA-QDa) analyses. We predicted protein structures, compared the predictions by multivariate analysis methods and found a striking correlation between BEA/ENN-producing profiles and ESYN1 three-dimensional structures. Structural differences in the β strand's Asn789-Ala793 and His797-Asp802 portions of the amino acid adenylation domain can be used to distinguish BEA/ENN-producing Fusarium isolates from those that produce only ENN.
外文关键词：enniatin；beauvericin；ESYN1；mycotoxins；Fusarium；homology modelling；multivariate analysis；backbone angles
作者：Liuzzi, VC；Mirabelli, V；Cimmarusti, MT；Haidukowski, M；Leslie, JF；Logrieco, AF；Caliandro, R；Fanelli, F；Mule, G
作者单位：CNR
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：2
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