用嗜吡啶红球菌株生物降解乙醇副产物中的黄曲霉毒素B1

Biological degradation of aflatoxin B (1) by a Rhodococcus pyridinivorans strain in by-product of bioethanol

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中文摘要:干酒糟可溶物(DDGS),是生物乙醇副产品中单独的、干燥的固体部分,常用作动物饲料。因监管问题玉米粒受黄曲霉毒素B1污染会影响以玉米为原料的生物乙醇生产。在预防无效的情况下,可能解决黄曲霉毒素污染问题的方案可能是对生物乙醇的生产原料或拟用作动物饲料的副产物进行解毒。本文研究并比较了为期三天的潜伏期间,一株嗜吡啶红球菌作为细菌接种物和细菌提取物,在液相和固相两个阶段降解黄曲霉毒素B1污染的生物乙醇副产品中的黄曲霉毒素的效率。本研究对通过嗜吡啶红球菌K408菌株降解源于生物乙醇生产副产品的动物饲料中的黄曲霉毒素B1有了早期了解。
外文摘要:Dried distillers grain with solubles (DDGS), as a separated and dried solid part of bioethanol by-products, is often utilized as animal feed. Aflatoxin B1 contamination of corn kernels may affect corn-based bioethanol production due to regulatory issues. In scenarios where prevention was not effective, possible solutions for aflatoxin contamination may be the detoxification of raw material of bioethanol production or the by-product, intended to be used as animal feed. In this paper we investigated and compared the aflatoxin degradation efficiency of a Rhodococcus pyridinivorans strain as bacterial inoculum and as bacterial extract in both the liquid and solid phases of an aflatoxin B1 contaminated bioethanol by-product (i.e. whole stillage) during a three-day incubation period. Results show that three-day microbial treatment cannot cause aflatoxin B1 degradation due to its tight binding into the solid stillage. Therefore longer, namely a twelve-day degradation experiment was performed in which the degradation profile of this strain was determined. Our results demonstrated that the aflatoxin content of the stillage significantly decreased between the 3rd and 7th days of the microbial reaction. More than two-thirds of the aflatoxin B1 content reacted during this time. The growth of bacteria was also monitored and statistically analysed. The exponential stage of biomass growth changed to a stagnant state after seven days incubation time, in harmony with the mycotoxin degradation rate. This work gives an early-state insight of aflatoxin B1 detoxification of animal feed, sourcing from bioethanol production as a by-product, by Rhodococcus pyridinivorans K408 bacterium strain.
外文关键词:Aflatoxin B-1;Biodegradation;Rhodococcus pyridinivorans K408;Stillage;DDGS
作者:Prettl, Z;Desi, E;Lepossa, A;Kriszt, B;Kukolya, J;Nagy, E
作者单位:Univ Pannonia
期刊名称:ANIMAL FEED SCIENCE AND TECHNOLOGY
期刊影响因子:1.713
出版年份:2017
出版刊次:-
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  1. 编译服务:农产品质量安全
  2. 编译者:虞德容
  3. 编译时间:2017-07-26