中文摘要：用杂色曲霉素（STC）-乙醇酸乙醚衍生物(STC-GE)偶联钥孔戚血蓝蛋白（抗原）构建了一种简单的制备抗STC的兔多克隆抗体的方法。这一新的分析工具能提高差异分析STC和黄曲霉毒素的可能性。
 
外文摘要：A simplified method to produce specific polyclonal rabbit antibodies against sterigmatocystin (STC) was established, using a STC-glycolic acid-ether derivative (STC-GE) conjugated to keyhole limpet haemocyanin (immunogen). The competitive direct enzyme immunoassay (EIA) established for STC had a detection limit (20% binding inhibition) of 130 pg ml−1. The test was highly specific for STC, with minor cross-reactivity with O-methylsterigmatocystin (OMSTC, 0·87%) and negligible reactivity with aflatoxins ( Significance and Impact of the StudyThe carcinogenic mycotoxin sterigmatocystin (STC) is produced by several Aspergillus species, either alone or together with aflatoxins. Here, we report a very simple and straightforward procedure to obtain highly sensitive and specific anti-STC antibodies, and their use in the first ever real STC-specific competitive direct enzyme immunoassay (EIA). In combination with a previous EIA for aflatoxins, this study for the first time demonstrates the potential of a STC/aflatoxin EIA pair for what is branded as ‘immunochemotaxonomic’ identification of mycotoxigenic Aspergillus species. This new analytical tool enhances analytical possibilities for differential analysis of STC and aflatoxins.
外文关键词：aflatoxin；Aspergillus；immunoassay；mycotoxin；polycolonal antibodies；sterigmatocystin
作者：Wegner, S；Bauer, JI；Dietrich, R；Martlbauer, E；Usleber, E；Gottschalk, C；Gross, M
作者单位：Justus Liebig Univ
期刊名称：LETTERS IN APPLIED MICROBIOLOGY
期刊影响因子：1.579
出版年份：2017
出版刊次：2
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